Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, College of Bioengineering at Chongqing University, Chongqing, China.
Key Laboratory for Biorheological Science and Technology of Ministry of Education, State and Local Joint Engineering Laboratory for Vascular Implants, College of Bioengineering at Chongqing University, Chongqing, China.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110187. doi: 10.1016/j.msec.2019.110187. Epub 2019 Sep 10.
It is widely accepted that surface biofunctional modification may be an effective approach to improve biocompatibility and confer new bioactive properties on biomaterials. In this work, mussel adhesive protein (MAP) was applied as a coating on 316 L stainless steel substrates (316 L SS) and stents, and then either immobilized VEGF or CD34 antibody were added to create biofunctional films. The properties of the MAP coating were characterized by scanning electron microscope (SEM), atomic force microscope (AFM) and a water contact angle test. Universal tensile testing showed that the MAP coating has adequate adhesion strength on a 316 L stainless steel material surface. Subsequent cytotoxicity and hemolysis rate tests showed that the MAP coatings have good biocompatibility. Moreover, using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysulfosussinimide (EDC/NHS) chemistry, VEGF and CD34 antibody were immobilized on the MAP coatings. The amount and immobilized yield of VEGF on the MAP coatings were analyzed by enzyme-linked immuno-assays (ELISA). Finally, an endothelial cells culture showed that the VEGF biofunctional film can promote the viability and proliferation of endothelial cells. An in vitro CD34 cells capturing test also verified the bioactive properties of the CD34 antibody coated stents. These results showed that the MAP coatings allowed effective biomolecule immobilization, providing a promising platform for vascular device modification.
人们普遍认为，表面生物功能修饰可能是一种提高生物相容性和赋予生物材料新生物活性的有效方法。在这项工作中，贻贝类黏附蛋白（MAP）被用作 316L 不锈钢基底（316L SS）和支架的涂层，然后添加固定化 VEGF 或 CD34 抗体以创建生物功能膜。MAP 涂层的特性通过扫描电子显微镜（SEM）、原子力显微镜（AFM）和水接触角测试进行了表征。万能拉伸试验表明，MAP 涂层在 316L 不锈钢材料表面具有足够的附着力。随后的细胞毒性和溶血率试验表明，MAP 涂层具有良好的生物相容性。此外，通过使用 N-（3-二甲基氨基丙基）-N'-乙基碳二亚胺盐酸盐和 N-羟基琥珀酰亚胺（EDC/NHS）化学，将 VEGF 和 CD34 抗体固定在 MAP 涂层上。通过酶联免疫吸附试验（ELISA）分析 MAP 涂层上 VEGF 的量和固定化产率。最后，内皮细胞培养表明，VEGF 生物功能膜可以促进内皮细胞的活力和增殖。体外 CD34 细胞捕获试验也验证了涂有 CD34 抗体的支架的生物活性。这些结果表明，MAP 涂层允许有效的生物分子固定化，为血管装置改性提供了一个有前途的平台。
