原发性肿瘤不同部位的免疫微环境与食管鳞癌临床结局的关系。
Relationship between the immune microenvironment of different locations in a primary tumour and clinical outcomes of oesophageal squamous cell carcinoma.
机构信息
Division of Pathology, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Kashiwa, Chiba, Japan.
Department of Gastroenterology and Gastrointestinal Oncology, National Cancer Center Hospital East, Kashiwa, Chiba, Japan.
出版信息
Br J Cancer. 2020 Feb;122(3):413-420. doi: 10.1038/s41416-019-0622-3. Epub 2019 Nov 25.
BACKGROUND
Tumour microenvironments can differ according to intratumoural locations. We investigated the immune status at different locations in primary tumours and its clinical significance in oesophageal squamous cell carcinoma (ESCC).
METHODS
The number of CD8 tumour-infiltrating immune cells (TIICs) and PD-1 TIICs, and PD-L1 expression on tumour cells (PD-L1) were immunohistochemically examined in the surface (Surf), centre (Cent) and invasive front (Inv) of tumours surgically resected from 192 patients with ESCC.
RESULTS
The PD-L1 rate was lower in Inv than in Cent (12.0% vs. 18.2%, P = 0.012), although the numbers of CD8 TIICs and PD-1 TIICs were comparable among intratumoural locations. High numbers of CD8 and PD-1 TIICs and positive PD-L1 were related to better overall survival (OS) only in Surf and Cent (CD8: P = 0.012 in Surf, 0.018 in Cent, and 0.165 in Inv; PD-1: P = 0.028 in Surf, 0.021 in Cent, and 0.208 in Inv; and PD-L1: 0.044 in Surf, 0.026 in Cent, and 0.718 in Inv). Positive PD-L1 in Surf and/or Cent but not in Inv demonstrated a strong tendency toward better OS (P = 0.053).
CONCLUSIONS
Immune microenvironments according to the intratumoural location have different effects on the survival of patients with ESCC.
背景
肿瘤微环境可因肿瘤内位置而异。我们研究了原发性肿瘤不同部位的免疫状态及其在食管鳞癌（ESCC）中的临床意义。
方法
对 192 例 ESCC 患者手术切除的肿瘤表面（Surf）、中心（Cent）和侵袭前缘（Inv）部位，采用免疫组织化学方法检测 CD8 肿瘤浸润免疫细胞（TIIC）和 PD-1 TIIC 的数量以及肿瘤细胞上的 PD-L1 表达（PD-L1）。
结果
尽管肿瘤内各部位的 CD8 TIIC 和 PD-1 TIIC 数量相当，但 Inv 处的 PD-L1 率低于 Cent（12.0% vs. 18.2%，P=0.012）。高数量的 CD8 和 PD-1 TIIC 以及阳性 PD-L1 仅与 Surf 和 Cent 处的总生存期（OS）相关（CD8：Surf 处 P=0.012，Cent 处 P=0.018，Inv 处 P=0.165；PD-1：Surf 处 P=0.028，Cent 处 P=0.021，Inv 处 P=0.208；PD-L1：Surf 处 P=0.044，Cent 处 P=0.026，Inv 处 P=0.718）。Surf 和/或 Cent 处的阳性 PD-L1 但 Inv 处阴性与更好的 OS 呈强烈趋势相关（P=0.053）。
结论
根据肿瘤内位置的免疫微环境对 ESCC 患者的生存有不同的影响。
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