Wang Xiao-Qiang, Kim Kyu-Won, Chu Sang-Ho, Phitaktansakul Rungnapa, Park Sang-Won, Chung Ill-Min, Lee Young-Sang, Park Yong-Jin
Department of Plant Resources, College of Industrial Science and Center of Crop Breeding on Omics and Artificial Intelligence, Kongju National University, Yesan 32439, Republic of Korea.
DiaCarta Inc. Yangzi Sci-Tech Innovation Center, Floor 21, Building A, No. 211 Pubin Road, Pukou District, Nanjing, Jiangsu 210000, China.
ACS Omega. 2019 Nov 7;4(21):19358-19365. doi: 10.1021/acsomega.9b02754. eCollection 2019 Nov 19.
Squalene is an isoprenoid compound that acts as the intermediate metabolite in cholesterol synthesis. Squalene is not very susceptible to peroxidation, and it quenches singlet oxygen in the skin, which is caused by UV exposure and other ionizing radiation sources. Squalene is a precursor to phytosterol synthesis, and it has been widely studied for its ability to reduce oxidation, cancer activity, and cholesterol levels. We performed a genome-wide association study for squalene in rice using 1.6 million high-quality SNPs extracted from 295 accessions' resequencing data. The candidate gene locus Os09g0319800-an orthologue of terpene synthase in -showed up as the most likely candidate gene amongst the identified loci. Nucleotide variations in the promoter were associated with squalene content variations within the group. The results of this study can provide clues for understanding the mechanisms of squalene biosynthesis in rice.
角鲨烯是一种类异戊二烯化合物，在胆固醇合成中作为中间代谢产物。角鲨烯不太容易发生过氧化反应，它能淬灭皮肤中由紫外线照射和其他电离辐射源引起的单线态氧。角鲨烯是植物甾醇合成的前体，因其具有降低氧化、抗癌活性和胆固醇水平的能力而受到广泛研究。我们利用从295份材料的重测序数据中提取的160万个高质量单核苷酸多态性（SNP），对水稻中的角鲨烯进行了全基因组关联研究。候选基因位点Os09g0319800（一种萜烯合酶的直系同源基因）在已鉴定的位点中显示为最有可能的候选基因。启动子中的核苷酸变异与该组内的角鲨烯含量变异相关。本研究结果可为理解水稻角鲨烯生物合成机制提供线索。
