Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
Department of Biochemistry, Federal University of Technology, Akure, Nigeria; Department of Biomedical Technology, Federal University of Technology, Akure, Nigeria.
Biomed J. 2019 Oct;42(5):317-327. doi: 10.1016/j.bj.2019.01.005. Epub 2019 Oct 31.
In this study, gallic acid (GA) and its polymeric form-tannic acid (TA) which are two phenolic acids found abundantly distributed in plant food sources were investigated for their influence on therapeutic properties of acarbose (AC) in vitro and in vivo in Drosophila melanogaster.
Combinations of AC and GA or TA were assessed for their alpha-glucosidase and alpha-amylase inhibitory effects as markers of anti-hyperglycemic properties, as well as their free radicals scavenging, Fe chelating and malondialdehyde (MDA) inhibitory effects (in vitro). Furthermore, wild type D. melanogaster cultures were raised on diets containing AC, GA, TA and their various combinations for seven days. Thereafter, flies were homogenized and glucose concentrations, alpha-glucosidase and alpha-amylase activities, as well as reactive oxygen species (ROS) and total thiol levels were determined.
The results showed that GA and TA up to 5 mg/ml significantly (p < 0.05) increased the enzymes' inhibitory effects and antioxidant properties of AC in vitro. Also, there was significant reduction in glucose concentration, enzyme activities and ROS level in D. melanogaster fed diets supplemented with phenolic acids and acarbose.
These bioactive compounds-drug interactions provide useful information on improving the therapeutic properties of acarbose especially in its use as an antidiabetic drug.
在这项研究中，研究了广泛存在于植物性食物来源中的两种酚酸——没食子酸（GA）及其聚合形式——单宁酸（TA），以研究它们对阿卡波糖（AC）在体外和黑腹果蝇体内的治疗特性的影响。
评估了 AC 与 GA 或 TA 的组合对α-葡萄糖苷酶和α-淀粉酶抑制作用的影响，作为抗高血糖特性的标志物，以及它们的自由基清除、Fe 螯合和丙二醛（MDA）抑制作用（体外）。此外，将野生型黑腹果蝇培养物饲养在含有 AC、GA、TA 及其各种组合的饮食中 7 天。然后，将果蝇匀浆，并测定葡萄糖浓度、α-葡萄糖苷酶和α-淀粉酶活性以及活性氧（ROS）和总巯基水平。
结果表明，GA 和 TA 高达 5mg/ml 时，可显著（p<0.05）增加 AC 在体外的酶抑制作用和抗氧化特性。此外，在补充了酚酸和阿卡波糖的饮食中，黑腹果蝇的葡萄糖浓度、酶活性和 ROS 水平显著降低。
这些生物活性化合物-药物相互作用提供了有关改善阿卡波糖治疗特性的有用信息，特别是在将其用作抗糖尿病药物方面。
