Department of General Surgery, Tungwah Hospital of Sun Yat-Sen University, Dongguan, Guangdong, China.
Department of Breast Cancer, Cancer Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
Technol Cancer Res Treat. 2019 Jan-Dec;18:1533033819892264. doi: 10.1177/1533033819892264.
Numerous researches have demonstrated that miR-142-5p plays significant roles in several cancers, although the functional characteristic of miR-142-5p in breast cancer has not been determined. This study is designed to explore the biological significance of miR-142-5p in breast cancer clinical implication and mechanism of action.
The differential expression patterns of miR-142-5p and Sorbin and SH3 domain-containing protein 1 and correlations between them and clinical significances were analyzed based on data from database. The expression levels of miR-142-5p in breast cancer cells were detected using quantitative real-time polymerase chain reaction. Cell counting kit-8, transwell, and wound healing assays were used to explore the potential functions of miR-142-5p in breast cancer cells. In addition, bioinformatics prediction analysis and luciferase reporter assay were utilized to predict and identify the potential target gene of miR-142-5p. A rescue experiment was conducted by transfecting miR-142-5p inhibitors and si-Sorbin and SH3 domain-containing protein 1 into cells to explore miR-142-5p/Sorbin and SH3 domain-containing protein 1 pairs on breast cancer cells behaviors.
The analysis results showed that miR-142-5p was highly expressed in patients with breast cancer, while Sorbin and SH3 domain-containing protein 1 presented a trend of low expression. The clinical significances analysis suggested that the overexpression of miR-142-5p is closely correlated with metastasis, while low expression of Sorbin and SH3 domain-containing protein 1 is correlated with clinicopathological characteristics and poor overall survival in patients with breast cancer. exploration, the expression of miR-142-5p was upregulated in breast cancer cells and inhibition of miR-142-5p expression significantly reduced the proliferation, invasion, and migration of breast cancer cells. Through rescue experiments, breast cancer cells proliferation, invasion, and migration reduction induced by silencing of miR-142-5p were reversed via knockdown Sorbin and SH3 domain-containing protein 1.
Our findings insinuate that miR-142-5p functions as a positive regulator of promoting breast cancer cells biological behaviors and clinical metastasis, possibly regulated by targeting Sorbin and SH3 domain-containing protein 1, thus providing valuable information in the development of preventive or even therapeutic strategies for utilizing miR-142-5p as a promising target.
许多研究表明 miR-142-5p 在多种癌症中发挥重要作用，尽管其在乳腺癌中的功能特征尚未确定。本研究旨在探讨 miR-142-5p 在乳腺癌临床意义和作用机制中的生物学意义。
基于数据库中的数据，分析 miR-142-5p 和 Sorbin 和 SH3 结构域蛋白 1 的差异表达模式及其与临床意义的相关性。使用实时定量聚合酶链反应检测乳腺癌细胞中 miR-142-5p 的表达水平。细胞计数试剂盒-8、Transwell 和划痕愈合实验用于探索 miR-142-5p 在乳腺癌细胞中的潜在功能。此外，通过生物信息学预测分析和荧光素酶报告基因实验预测和鉴定 miR-142-5p 的潜在靶基因。通过转染 miR-142-5p 抑制剂和 si-Sorbin 和 SH3 结构域蛋白 1 进入细胞，进行拯救实验，以探索 miR-142-5p/Sorbin 和 SH3 结构域蛋白 1 对乳腺癌细胞行为的影响。
分析结果表明，miR-142-5p 在乳腺癌患者中高表达，而 Sorbin 和 SH3 结构域蛋白 1 呈低表达趋势。临床意义分析表明，miR-142-5p 的高表达与转移密切相关，而 Sorbin 和 SH3 结构域蛋白 1 的低表达与乳腺癌患者的临床病理特征和总体生存不良相关。进一步研究表明，miR-142-5p 在乳腺癌细胞中表达上调，抑制 miR-142-5p 表达可显著降低乳腺癌细胞的增殖、侵袭和迁移。通过拯救实验，沉默 miR-142-5p 诱导的乳腺癌细胞增殖、侵袭和迁移减少可通过敲低 Sorbin 和 SH3 结构域蛋白 1 逆转。
我们的研究结果表明，miR-142-5p 作为促进乳腺癌细胞生物学行为和临床转移的正向调节剂发挥作用，可能通过靶向 Sorbin 和 SH3 结构域蛋白 1 进行调节，从而为利用 miR-142-5p 作为有前途的治疗靶点提供有价值的信息。
