Institute for Cardiac Diagnostics and Therapy (IKDT), Moltkestr. 31, Berlin, Germany.
Division of Viral Gastroenteritis and Hepatitis Pathogens and Enteroviruses, Department of Infectious Diseases, Robert Koch Institute, Berlin, Germany.
ESC Heart Fail. 2023 Dec;10(6):3410-3418. doi: 10.1002/ehf2.14523. Epub 2023 Sep 7.
Inflammation of the heart is a complex biological and pathophysiological response of the immune system to a variety of injuries leading to tissue damage and heart failure. MicroRNAs (miRNAs) emerge as pivotal players in the development of numerous diseases, suggesting their potential utility as biomarkers for inflammation and as viable candidates for therapeutic interventions. The primary aim of this investigation was to pinpoint and assess particular miRNAs in individuals afflicted by virus-negative inflammatory dilated cardiomyopathy (DCMi).
The study involved the analysis of 152 serum samples sourced from patients diagnosed with unexplained heart failure through endomyocardial biopsy. Among these samples, 38 belonged to DCMi patients, 24 to DCM patients, 44 to patients displaying inflammation alongside diverse viral infections, and 46 to patients solely affected by viral infections without concurrent inflammation. Additionally, serum samples from 10 healthy donors were included. The expression levels of 754 distinct miRNAs were evaluated using TaqMan OpenArray. MiR-1, miR-23, miR-142-5p, miR-155, miR-193, and miR-195 exhibited exclusive down-regulation solely in DCMi patients (P < 0.005). These miRNAs enabled effective differentiation between individuals with inflammation unlinked to viruses (DCMi) and all other participant groups (P < 0.005), boasting a specificity surpassing 86%.
The identification of specific miRNAs offers a novel diagnostic perspective for recognizing intramyocardial inflammation within virus-negative DCMi patients. Furthermore, these miRNAs hold promise as potential candidates for tailored therapeutic strategies in the context of virus-negative DCMi.
心肌炎症是免疫系统对各种导致组织损伤和心力衰竭的损伤的复杂生物和病理生理反应。 microRNAs（miRNAs）作为许多疾病发展的关键参与者出现，这表明它们作为炎症的潜在生物标志物和可行的治疗干预候选物具有潜力。本研究的主要目的是确定并评估患有病毒阴性炎症性扩张型心肌病（DCMi）的个体中的特定 miRNAs。
该研究分析了通过心内膜心肌活检诊断为不明原因心力衰竭的 152 份血清样本。在这些样本中，38 份属于 DCMi 患者，24 份属于 DCM 患者，44 份属于同时伴有多种病毒感染的炎症患者，46 份属于仅受病毒感染而无并发炎症的患者。此外，还包括了 10 名健康供体的血清样本。使用 TaqMan OpenArray 评估了 754 种不同 miRNAs 的表达水平。miR-1、miR-23、miR-142-5p、miR-155、miR-193 和 miR-195 在 DCMi 患者中仅表现出特异性下调（P<0.005）。这些 miRNAs 能够有效地将无病毒相关炎症的个体（DCMi）与所有其他参与者群体区分开来（P<0.005），特异性超过 86%。
特异性 miRNAs 的鉴定为识别病毒阴性 DCMi 患者的心肌内炎症提供了新的诊断视角。此外，这些 miRNAs 有希望成为病毒阴性 DCMi 中定制治疗策略的潜在候选物。
