细胞外囊泡的生成、纯化和工程及其在生物医学中的应用。
Generation, purification and engineering of extracellular vesicles and their biomedical applications.
机构信息
Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99202, USA.
Department of Pharmaceutical Sciences, College of Pharmacy and Pharmaceutical Sciences, Washington State University, Spokane, WA 99202, USA.
出版信息
Methods. 2020 May 1;177:114-125. doi: 10.1016/j.ymeth.2019.11.012. Epub 2019 Nov 30.
Abstract
Extracellular vesicles (EVs), derived from cell membranes, demonstrate the potential to be excellent therapeutics and drug carriers. Although EVs are promising, the process to develop high-quality and scalable EVs for their translation is demanding. Within this research, we analyzed the production of EVs, their purification and their post-bioengineering, and we also discussed the biomedical applications of EVs. We focus on the developments of methods in producing EVs including biological, physical, and chemical approaches. Furthermore, we discuss the challenges and the opportunities that arose when we translated EVs in clinic. With the advancements in nanotechnology and immunology, genetically engineering EVs is a new frontier in developing new therapeutics in order to tailor to individuals and different disease stages in treatments of cancer and inflammatory diseases.
摘要
细胞外囊泡 (EVs) 来源于细胞膜，具有成为优秀治疗药物和药物载体的潜力。尽管 EVs 很有前途，但要将其开发为高质量且可扩展的 EV 以进行转化，这一过程极具挑战性。在这项研究中，我们分析了 EV 的生产、纯化及其生物工程后处理，还讨论了 EV 的生物医学应用。我们专注于生产 EV 的方法的发展，包括生物、物理和化学方法。此外，我们还讨论了在将 EV 转化为临床应用时出现的挑战和机遇。随着纳米技术和免疫学的进步，对 EV 进行基因工程是开发新治疗方法的新前沿，旨在针对个体和癌症及炎症性疾病治疗的不同阶段进行定制。
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