Gómez Jessica, Simirgiotis Mario J, Lima Beatriz, Gamarra-Luques Carlos, Bórquez Jorge, Caballero Duilio, Feresin Gabriela Egly, Tapia Alejandro
Instituto de Biotecnología-Instituto de Ciencias Básicas, Universidad Nacional de San Juan, Av. Libertador General San Martín 1109 (O), San Juan CP 5400, Argentina.
CONICET (Consejo Nacional de Ciencia y Tecnología), CABA, Buenos Aires C1405DJR, Argentina.
Antioxidants (Basel). 2019 Nov 28;8(12):593. doi: 10.3390/antiox8120593.
The decoction of is used in traditional medicine of South America as hypocholesterolemic, balsamic, and expectorant; but it is also useful for the prevention of hepatitis, renal insufficiency, and diabetes, and is used as digestive. A lyophilized decoction from the aerial parts of this plant (TLD) collected in San Juan (TLDSJ) and Mendoza (TLDM) provinces (Argentina) and one collection from Antofagasta, Chile (TLDCH) were characterized regarding antioxidant and antibacterial activities, phenolics and flavonoids content, and ultrahigh resolution liquid chromatography Orbitrap MS analysis UHPLC-PDA-OT-MS/MS metabolite profiling. The antioxidant properties were carried out "in vitro" using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and trolox equivalent antioxidant activity (TEAC) methods, ferric-reducing antioxidant power (FRAP), and lipoperoxidation in erythrocytes (LP). The antibacterial activity was evaluated following the Clinical and Laboratory Standards Institute (CLSI) rules. TLDSJ, TLDM, and TLDCH displayed a strong DPPH scavenging activity (EC = 42, 41.6, and 43 µg/mL, respectively) and inhibition of lipoperoxidation in erythrocytes (86-88% at 250 µg TLD/mL), while a less effect in the FRAP and TEACantioxidant assays was found. Additionally, the decoctions showed a content of phenolics compounds of 94 mg gallic acid equivalents (GAE)/g, 185 GAE/g, and 64 GAE/g, for TLDSJ, TLDM, and TLDCH samples, respectively. Regarding the flavonoid content, the Chilean sample was highlighted with 19 mg quercetin equivalents (QE)/g. In this work, several phenolic compounds, including sesquiterpenes, flavonoids, and phenolic acids, were rapidly identified in TLDSJ, TLDM, and TLDCH extracts by means UHPLC-PDA-OT-MS/MS for the first time, which gave a first scientific support to consider this medicinal decoction from both countries as a valuable source of metabolites with antioxidant effects, some with outstanding potential to improve human health.
在南美洲传统医学中，[植物名称]的煎剂被用作降胆固醇、香脂和祛痰剂；但它对预防肝炎、肾功能不全和糖尿病也有作用，还可作为消化剂。对采自阿根廷圣胡安省（TLDSJ）和门多萨省（TLDM）的该植物地上部分的冻干煎剂以及采自智利安托法加斯塔的一批煎剂（TLDCH）进行了抗氧化和抗菌活性、酚类和黄酮类含量以及超高分辨率液相色谱-轨道阱质谱分析（UHPLC-PDA-OT-MS/MS）代谢物谱分析。使用2,2-二苯基-1-苦基肼（DPPH）和特洛克斯等效抗氧化活性（TEAC）方法、铁还原抗氧化能力（FRAP）以及红细胞中的脂质过氧化（LP）“体外”测定抗氧化性能。按照临床和实验室标准协会（CLSI）规则评估抗菌活性。TLDSJ、TLDM和TLDCH表现出很强的DPPH清除活性（EC分别为42、41.6和43 µg/mL）以及对红细胞脂质过氧化的抑制作用（在250 µg TLD/mL时为86 - 88%），而在FRAP和TEAC抗氧化测定中效果较差。此外，TLDSJ、TLDM和TLDCH样品的酚类化合物含量分别为94毫克没食子酸当量（GAE）/克、185 GAE/克和64 GAE/克。关于黄酮类含量，智利样品突出，为19毫克槲皮素当量（QE）/克。在这项工作中，首次通过UHPLC-PDA-OT-MS/MS在TLDSJ、TLDM和TLDCH提取物中快速鉴定出几种酚类化合物，包括倍半萜、黄酮类和酚酸，这为将来自这两个国家的这种药用煎剂视为具有抗氧化作用的代谢物的宝贵来源提供了首个科学依据，其中一些具有改善人类健康的巨大潜力。
