Agricultural Faculty, Department of Food Engineering, Selçuk University, 42130, Konya, Turkey.
Probiotics Antimicrob Proteins. 2020 Jun;12(2):747-755. doi: 10.1007/s12602-019-09621-x.
The present study aimed to determine the effects of combined usage of β-cyclodextrin with whey protein isolate and sodium caseinate on the microencapsulation of Bifidobacterium bifidum-BB12 by spray drying.From the results, the highest count of B. bifidum was provided by whey protein isolate as 8.62 log CFU/g. The increasing concentration of β-cyclodextrin considerably increases gastric and intestinal resistance to B. bifidum cells. In the gastric and intestinal test, the highest protection was determined in whey protein isolate substituted with 10% β-cyclodextrin with reduction rates of 0.98 and 3.30%, respectively. Moreover, free cells did not survive in the same gastric conditions. The lowest hygroscopicity was determined in whey protein isolate as 8.57%. It must be noted that increasing β-cyclodextrin concentration in carrier material combination led to an increase in hygroscopicity of microcapsules. In general, substitution with β-cyclodextrin increased the particle size of microparticles, and microcapsules produced with whey protein isolate had a smaller size than that of sodium caseinate.
本研究旨在通过喷雾干燥法确定β-环糊精与乳清蛋白分离物和酪蛋白酸钠联合使用对双歧杆菌-BB12 的微胶囊化的影响。结果表明，乳清蛋白分离物提供的双歧杆菌数量最高，为 8.62 log CFU/g。β-环糊精浓度的增加显著提高了双歧杆菌细胞的胃和肠道抗性。在胃和肠道试验中，用 10%β-环糊精替代乳清蛋白分离物时，保护率最高，分别为 0.98%和 3.30%。此外，游离细胞在相同的胃条件下无法存活。乳清蛋白分离物的吸湿性最低，为 8.57%。需要注意的是，载体材料组合中β-环糊精浓度的增加会导致微胶囊吸湿性增加。总的来说，β-环糊精的替代增加了微粒的粒径，并且用乳清蛋白分离物生产的微胶囊比用酪蛋白酸钠生产的微胶囊粒径更小。
