1Department of Zoonoses, Faculty of Veterinary Medicine, Cairo University, Box 12211, Giza, PO Egypt.
2Department of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Box 12211, Giza, PO Egypt.
Antimicrob Resist Infect Control. 2019 Dec 3;8:197. doi: 10.1186/s13756-019-0657-5. eCollection 2019.
Antimicrobial resistance has become one of the most severe global threats to human and veterinary Medicinstin is an effective therapeutic agent against multi-drug-resistant pathogens. However, the discovery of transferable plasmids that confer resistance to colistin ( has led to challenges in medical science. This study describes the role of wild birds in the harbouring and environmental spread of colistin-resistant bacteria, which could pose a potential hazard to human and animal health.
In total, 140 faecal samples from wild birds (migratory and resident birds) were tested. Twenty surface water samples were collected from the area in which wild bird trapping was conducted, and 50 human stool samples were collected from individuals residing near the surface water sources and farm buildings. Isolation and identification of and from the different samples were performed using conventional culture techniques and biochemical identification. PCR amplification of the genes was performed in all positive isolates. Sequencing of -1 genes from three randomly selected carrying mcr-1 isolates; wild birds, water and humans was performed.
The bacteriological examination of the samples showing isolates of , , and . The results of multiplex PCR of the genes revealed that was the most prevalent gram-negative bacterium harbouring the genes, whereas a low prevalence was observed for . The prevalence of 1 in resident birds, migratory birds, water sources and humans were 10.4, 20,16.6 and 9.6% while the prevalence of -2 were 1.4, 3.6, 11.1 and 9.6%, respectively. Sequencing of the gene from the three carrying - isolates indicated a possible correlation between the wild bird and surface water isolates.
The detection of -1-positive bacteria in wild birds in Egypt indicates the possible environmental dissemination of this gene through bird activity. The impact of the interaction between domestic and wild animals on public health cannot be overlooked.
抗菌药物耐药性已成为人类和兽医医学面临的最严重的全球性威胁之一。黏菌素是一种对抗多药耐药病原体的有效治疗药物。然而，发现能够赋予对黏菌素耐药性的可转移质粒，这给医学科学带来了挑战。本研究描述了野生鸟类在携带和环境传播耐黏菌素细菌方面的作用，这可能对人类和动物健康构成潜在威胁。
共检测了 140 份来自野生鸟类（候鸟和留鸟）的粪便样本。从野生鸟类捕捉区采集了 20 份地表水样本，从居住在地表水水源和农场建筑物附近的个体中采集了 50 份人粪便样本。使用常规培养技术和生化鉴定从不同样本中分离和鉴定 。对所有阳性分离物进行 基因的 PCR 扩增。从携带 mcr-1 的三个随机选择的 携带菌株 - 野生鸟类、水和人类中进行了 -1 基因的测序。
样本的细菌学检查显示出 、 、 和 的分离物。 基因的多重 PCR 结果显示， 是携带 基因的最常见革兰氏阴性菌，而 的流行率较低。常驻鸟类、候鸟、水源和人类中 1 的流行率分别为 10.4%、20%、16.6%和 9.6%，而 -2 的流行率分别为 1.4%、3.6%、11.1%和 9.6%。从携带 - 的三个 携带菌株中 基因的测序表明，野生鸟类和地表水分离株之间可能存在相关性。
在埃及的野生鸟类中检测到 -1 阳性细菌表明，该基因可能通过鸟类活动在环境中传播。不能忽视家养和野生动物之间相互作用对公共卫生的影响。
