The Edison Family Center for Genome Sciences and Systems Biology, St. Louis, USA.
Center for Gut Microbiome and Nutrition Research, St. Louis, USA.
Microbiome. 2019 Dec 12;7(1):158. doi: 10.1186/s40168-019-0769-4.
Given the increasing use of gnotobiotic mouse models for deciphering the effects of human microbial communities on host biology, there is a need to develop new methods for characterizing these animals while maintaining their isolation from environmental microbes. We describe a method for performing open-circuit indirect calorimetry on gnotobiotic mice colonized with gut microbial consortia obtained from different human donors. In this illustrative case, cultured collections of gut bacterial strains were obtained from obese and lean co-twins. The approach allows microbial contributions to host energy homeostasis to be characterized.
鉴于无菌动物模型在破译人类微生物群落对宿主生物学影响方面的应用日益增多，因此需要开发新的方法来对这些动物进行特征描述，同时保持其与环境微生物的隔离。我们描述了一种对定植有来自不同供体的肠道微生物群落的无菌动物进行开路间接测热的方法。在这个说明性案例中，从肥胖和瘦的同卵双胞胎中获得了肠道细菌菌株的培养物集合。该方法可以对微生物对宿主能量平衡的贡献进行特征描述。
