Department of Senior Healthcare, BK21 Plus Program, Graduate School, Eulji University, Daejeon 34824.
Happybio R&D center, Happybio, Cheongju, Chungcheongbuk-do 28101.
Int J Med Sci. 2019 Nov 8;16(12):1604-1613. doi: 10.7150/ijms.37854. eCollection 2019.
Koehne (CS) has been used in a traditional oriental medicine for treating throat diseases, anaphylaxis, viral infection, and inflammation. This study investigated the underlying mechanism of anti-allergic effect of CS. Leaves of CS plants were dried, powdered, and then underwent extraction with DMSO. Both ELISA and western blotting were performed to evaluate cytokine concentration and the expression and activation of filaggrin and JNK. Five-week-old female NC/Nga mice were used as an AD-like mouse model by treating them with 2,4-dinitrochlorobenzene (DNCB). The secretion of TARC, MCP-1, and IL-8 is increased by TNF-α and IFN-γ in HaCaT cells, and CS extract inhibited the increased production of TARC, MCP-1, and IL-8. TNF-α and IFN-γ suppressed filaggrin expression by activating JNK. CS extract recovered the expression of filaggrin decreased by TNF-α and IFN-γ by blocking the activation of JNK. experiment, CS administration reduced thickening of the epidermis and infiltration of inflammatory cells into the dermis as compared to DNCB treatment. Moreover, the decrease of filaggrin expression due to DNCB treatment was recovered by CS administration. The serum IgE level was decreased by CS treatment. The levels of IL-4, IL-5, IL-13 and eotaxin in mouse splenocytes increased after treatment with concanavalin A, and the secretions of IL-4, IL-5, IL-13 and eotaxin were lower in the CS-treated group than in the DNCB group. These results may contribute to the development of a CS-based drug for the treatment of atopic dermatitis.
柯恩（CS）在传统东方医学中用于治疗喉咙疾病、过敏反应、病毒感染和炎症。本研究旨在探讨 CS 的抗过敏作用机制。CS 植物的叶子被干燥、粉碎，然后用 DMSO 进行提取。通过 ELISA 和 Western blot 分析来评估细胞因子浓度以及丝聚蛋白和 JNK 的表达和激活。将 5 周龄雌性 NC/Nga 小鼠用 2,4-二硝基氯苯（DNCB）处理作为 AD 样小鼠模型。TNF-α 和 IFN-γ 可增加 HaCaT 细胞中 TARC、MCP-1 和 IL-8 的分泌，CS 提取物可抑制 TARC、MCP-1 和 IL-8 的产生增加。TNF-α 和 IFN-γ 通过激活 JNK 抑制丝聚蛋白的表达。CS 提取物通过阻断 JNK 的激活恢复了 TNF-α 和 IFN-γ 降低的丝聚蛋白表达。实验结果表明，与 DNCB 处理相比，CS 给药可减少表皮增厚和真皮炎症细胞浸润。此外，CS 给药可恢复由于 DNCB 处理导致的丝聚蛋白表达减少。CS 处理降低了血清 IgE 水平。刀豆蛋白 A 处理后，小鼠脾细胞中 IL-4、IL-5、IL-13 和 eotaxin 的水平增加，CS 处理组的 IL-4、IL-5、IL-13 和 eotaxin 分泌低于 DNCB 组。这些结果可能有助于开发基于 CS 的药物来治疗特应性皮炎。
