用于纳米毒理学研究的荧光标记纳米纤维素的开发与表征
Development & Characterization of Fluorescently Tagged Nanocellulose for Nanotoxicological Studies.
作者信息
Salari Maryam, Bitounis Dimitrios, Bhattacharya Kunal, Pyrgiotakis Georgios, Zhang Zhenyuan, Purington Emilia, Gramlich William, Grondin Yohann, Rogers Rick, Bousfield Douglas, Demokritou Philip
机构信息
Center for Nanotechnology and Nanotoxicology, Harvard T. H. Chan School of Public Health, Boston, MA, 07016, USA.
Department of Chemical and Biological Engineering, University of Maine, Orono ME 04469 USA.
出版信息
Environ Sci Nano. 2019 May 1;6(5):1516-1526. doi: 10.1039/C8EN01381K. Epub 2019 Apr 10.
The rapid adoption of nanocellulose-based engineered nanomaterials (CNM) by many industries generates environmental health and safety (EHS) concerns. This work presents the development of fluorescently tagged CNM which can be used to study their interactions with biological systems. Specifically, cellulose nano-fibrils and cellulose nano-crystals with covalently attached fluorescein isothiocyanate (FITC) molecules on their surface were synthesized. The fluorescence of the FITC-tagged materials was assessed along with potential FITC detachment under pH conditions encountered in the human gastrointestinal tract, in intracellular compartments, and in cell culture media. Finally, the potential cytotoxicity due to the presence of FITC molecules on the surface of CNM was assessed using a cellular gut epithelium model. The results showed that neither FITC-CNF nor FITC-CNC were cytotoxic and that they have a comparable bioactivity to their untagged counterparts, rendering them suitable for biological studies.
许多行业对基于纳米纤维素的工程纳米材料（CNM）的迅速采用引发了环境健康与安全（EHS）方面的担忧。这项工作展示了荧光标记的CNM的开发，其可用于研究它们与生物系统的相互作用。具体而言，合成了表面共价连接有异硫氰酸荧光素（FITC）分子的纤维素纳米纤维和纤维素纳米晶体。评估了FITC标记材料的荧光以及在人类胃肠道、细胞内区室和细胞培养基中遇到的pH条件下FITC的潜在脱离情况。最后，使用细胞肠道上皮模型评估了由于CNM表面存在FITC分子而导致的潜在细胞毒性。结果表明，FITC-CNF和FITC-CNC均无细胞毒性，并且它们与其未标记的对应物具有相当的生物活性，使其适用于生物学研究。
Zotero 插件