Institute of Animal Nutrition, Sichuan Agricultural University, and Key Laboratory of Animal Disease Resistance Nutrition Ministry of Education, Chengdu, Sichuan 611130, People's Republic of China.
Food Funct. 2020 Jan 29;11(1):585-595. doi: 10.1039/c9fo00627c.
The physicochemical characteristics of beta-glucans determine the immune responses of the intestines and whole body. It is hypothesized that glucans with different molecular weights have diverse modes of action on LPS-mediated immune activity. This study aimed to verify the immune-modulatory effects of two types of beta-glucans in LPS-induced weaned piglets. The results indicated that dietary beta-glucan supplementation could prevent losses in body weight gain caused by LPS challenge. Supplementation with different molecular weights of beta-glucans decreased the production of IL-1β and TNF-α and increased IL-10 production, which is likely associated with key factors such as TLR4 and NF-κB. High-molecular-weight beta-glucans seemed to have a strong functional capacity to modulate the innate immune response through the Dectin-1 receptor. Therefore, the results indicate that supplementing piglets with Agrobacterium sp. ZX09 beta-glucans inhibits LPS-mediated depression in the growth performance and plays a protective role during LPS challenge possibly via the Dectin-1 receptor and the TLR4/NF-κB pathway. The results reveal the potential therapeutic activity of purified Agrobacterium sp. ZX09 beta-glucan following experimental LPS infusion.
β-葡聚糖的理化特性决定了其对肠道和全身的免疫反应。有假说认为，具有不同分子量的葡聚糖对 LPS 介导的免疫活性具有不同的作用模式。本研究旨在验证两种β-葡聚糖在 LPS 诱导的断奶仔猪中的免疫调节作用。结果表明，日粮β-葡聚糖的添加可以预防 LPS 挑战引起的体重增长损失。不同分子量的β-葡聚糖的添加降低了 IL-1β和 TNF-α的产生，增加了 IL-10 的产生，这可能与 TLR4 和 NF-κB 等关键因素有关。高分子量的β-葡聚糖似乎通过 Dectin-1 受体具有很强的调节先天免疫反应的功能能力。因此，结果表明，向仔猪补充农杆菌 sp. ZX09β-葡聚糖可抑制 LPS 介导的生长性能下降，并在 LPS 挑战期间发挥保护作用，这可能是通过 Dectin-1 受体和 TLR4/NF-κB 途径。结果揭示了经实验性 LPS 输注后，纯化的农杆菌 sp. ZX09β-葡聚糖的潜在治疗活性。
