Department of Dietetics, Institute of Human Nutrition Sciences, Warsaw University of Life Sciences, Nowoursynowska 159c, 02-776 Warsaw, Poland.
Department of Physiological Sciences, Institute of Veterinary Medicine, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland.
Int J Mol Sci. 2021 Apr 25;22(9):4485. doi: 10.3390/ijms22094485.
The incidence of Crohn's disease (CD) is increasing worldwide, and it has currently become a serious public health issue in society. The treatment of CD continues throughout a patient's lifetime, and therefore, it is necessary to develop new, effective treatment methods, including dietotherapy. The present study aimed to determine the effects of consumption of oat beta-glucans with different molar mass on colon inflammation () in the early stages of 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced CD in an animal model.
Sprague-Dawley rats (control and TNBS-induced CD) were divided into three dietary groups and fed for 3 days (reflecting acute inflammation) or 7 days (reflecting remission) with a feed containing 1% low (βGl) or high (βGh) molar mass oat beta-glucan or a feed without this polysaccharide. The level of colon inflammatory markers and the expression of cytokines and their receptor genes were measured by ELISA and RT-PCR methods, respectively.
Acute inflammation or remission (3 or 7 days after TNBS administration, respectively) stages of experimentally induced CD were characterized by an increase in the level of inflammatory markers (IL-1, IL-6, IL-10, IL-12, TNF-α, CRP, MPO, COX, and PGE2) and the disruption of some cytokine signaling pathways as well as macro- and microscopic changes of colon tissue. The consumption of oat beta-glucans reduced the level of inflammatory markers and recovered the signaling pathways and histological changes, with stronger effects of βGl after 7 days of .
Dietary oat beta-glucans can reduce at the molecular and organ level and accelerate CD remission.
克罗恩病（CD）的发病率在全球范围内呈上升趋势，目前已成为社会严重的公共卫生问题。CD 的治疗贯穿患者的一生，因此，有必要开发新的、有效的治疗方法，包括饮食疗法。本研究旨在确定不同摩尔质量的燕麦β-葡聚糖对 2,4,6-三硝基苯磺酸（TNBS）诱导的 CD 动物模型早期结肠炎症（）的影响。
将 Sprague-Dawley 大鼠（对照组和 TNBS 诱导的 CD 组）分为三组饮食组，分别用含有 1%低（βGl）或高（βGh）摩尔质量的燕麦β-葡聚糖或不含该多糖的饲料喂养 3 天（反映急性炎症）或 7 天（反映缓解）。通过 ELISA 和 RT-PCR 方法分别测量结肠炎症标志物的水平和细胞因子及其受体基因的表达。
实验诱导的 CD 的急性炎症或缓解（分别在 TNBS 给药后 3 或 7 天）阶段的特征是炎症标志物（IL-1、IL-6、IL-10、IL-12、TNF-α、CRP、MPO、COX 和 PGE2）水平升高，以及一些细胞因子信号通路的中断，以及结肠组织的宏观和微观变化。燕麦β-葡聚糖的摄入降低了炎症标志物的水平，并恢复了信号通路和组织学变化，βGl 在 7 天的摄入后效果更强。
膳食燕麦β-葡聚糖可降低分子和器官水平的，并加速 CD 缓解。
