Universidade Federal de Pernambuco, Departamento Ciências farmacêuticas, CEP: 50740-520, Recife, PE, Brazil.
Universidade Federal de Pernambuco, Departamento Ciências farmacêuticas, CEP: 50740-520, Recife, PE, Brazil.
Environ Toxicol Pharmacol. 2020 Apr;75:103313. doi: 10.1016/j.etap.2019.103313. Epub 2019 Dec 17.
In order to evaluate dermal absorption during typical working conditions in family farming, the amount of dimethoate on clothing and in the stratum corneum (SC) was measured in three rural workers. This was achieved by using cotton patches on the worker's clothes and SC quantification by the tape stripping approach. To mimic the above study, an in vitro approach was performed using Franz cells by applying dimethoate (0.4 and 1.8 μg) direct to pig skin or, on a section of cotton before contact with pig skin. The in vivo case results demonstrated the high levels and variability of agrochemicals to which these farmers are subjected, with the total potential dermal absorption between 0.87-2.85 mg/person/h and the estimated SC penetration factor (PF) between 0-54.0 and 0-28.9 % for the back of the neck and the arms respectively. This probably demonstrates the impact of correct protective clothing. For the in vitro study, the amount of pesticide retained in the SC was 52.63 ± 10.73and 135.15 ± 31.8 ng/cm after applying 0.4 and 1.8 μg of pesticide directly on SC, and demonstrated close agreement with the in vivo approach. Further studies performed with this and other pesticides with different characteristics will contribute to the understanding of their transport through the skin.
为了评估家庭农场典型工作条件下的皮肤吸收情况，对三名农村工人的衣物和角质层（SC）上的乐果含量进行了测量。这是通过在工人衣物上使用棉贴片以及通过胶带剥离法对 SC 进行定量来实现的。为了模拟上述研究，使用 Franz 细胞通过将乐果（0.4 和 1.8μg）直接施用于猪皮或在与猪皮接触之前在棉片上进行体外方法。体内情况表明，这些农民所接触的农药水平很高且具有变异性，总潜在皮肤吸收率在 0.87-2.85mg/人/h 之间，颈部和手臂背部的估计 SC 渗透因子（PF）分别在 0-54.0%和 0-28.9%之间。这可能表明了正确的防护服的影响。对于体外研究，将 0.4 和 1.8μg 的农药直接施用于 SC 后，SC 中残留的农药量分别为 52.63±10.73 和 135.15±31.8ng/cm，与体内方法非常吻合。使用这种和其他具有不同特性的农药进行进一步研究，将有助于了解它们通过皮肤的传输。
