单菌落裂解物中的质粒检测与大小测定
Plasmid detection and sizing in single colony lysates.
作者信息
Barnes W M
出版信息
Science. 1977 Jan 28;195(4276):393-4. doi: 10.1126/science.318764.
PMID:318764
Abstract
A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.
摘要
本文描述了一种简单且封闭的程序，用于快速检测类似于Col E1的质粒的存在及其大小。用牙签从琼脂平板上挑取细菌，用十二烷基硫酸盐裂解并加热，然后直接置于琼脂糖凝胶上进行电泳。
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