Department of Orthopedics, The First Affiliated Hospital of Jinan University, Guangzhou, 510630, China.
Department of Material Science and Engineering, Engineering Research Center of Artificial Organs and Materials, Jinan University, Guangzhou, 510632, China; Institute of Biomedical Engineering, College of Life Science and Technology, Jinan University, Guangzhou, 510632, China.
Carbohydr Polym. 2020 Feb 15;230:115710. doi: 10.1016/j.carbpol.2019.115710. Epub 2019 Dec 6.
It is urgently needed for effective treatments of extensive skin loss, wherein lack of angiogenesis is a major obstacle. In this study, we present a thermosensitive thiolated chitosan (CSSH) hydrogel conjugated with Histatin1 (Hst1) as a wound dressing to study its efficacy in enhancing the cell adhesion, spreading, migration, and angiogenesis. The composite hydrogels with gelation time of 5-7 min, showed a prolonged release of Hst1. Cell culture indicated that the adhesion, spreading, migration and tubule formation of HUVECs were promoted, especially for the Hst1-H group. The in vivo healing evaluation showed that the rate of recovery in Hst1-H group was increased to 84% at day 7, and the CD31 positive cells, vascular endothelial growth factor (VEGF) positive cells and aligned collagen fibers were significantly more than the controlled groups. Therefore, CSSH/Hst1 hydrogel is a promising candidate for wound healing by accelerating cell adhesion, migration and angiogenesis.
对于广泛皮肤缺失的有效治疗，缺乏血管生成是一个主要的障碍。在这项研究中，我们提出了一种基于壳聚糖的温敏水凝胶（CSSH）与Histatin1（Hst1）的复合水凝胶作为一种伤口敷料，以研究其在促进细胞黏附、扩散、迁移和血管生成方面的功效。该复合水凝胶的胶凝时间为 5-7 分钟，具有延长 Hst1 释放的效果。细胞培养表明，HUVECs 的黏附、扩散、迁移和管状结构形成得到了促进，特别是在 Hst1-H 组中。体内愈合评估表明，Hst1-H 组在第 7 天的恢复率提高到 84%，并且 CD31 阳性细胞、血管内皮生长因子（VEGF）阳性细胞和排列整齐的胶原纤维明显多于对照组。因此，CSSH/Hst1 水凝胶通过加速细胞黏附、迁移和血管生成，是一种有前途的伤口愈合候选材料。
