Vázquez-Sánchez Alma Yolanda, Aguilar-Zárate Pedro, Muñiz-Márquez Diana Beatriz, Wong-Paz Jorge Enrique, Rojas Romeo, Ascacio-Valdés Juan Alberto, Martínez-Ávila Guillermo Cristian G
Universidad Tecnológica de Xicotepec de Juárez Área Agroindustrial Alimentaria. Av, Universidad Tecnológica No. 1000, Tierra Negra, Xicotepec de Juárez, Puebla, C.P. 73080, Mexico.
Tecnológico Nacional de México, Campus Ciudad Valles, Departamento de Ingenierías, Carretera al Ingenio Plan de Ayala Km. 2, Colonia Vista Hermosa, Ciudad Valles, San Luis Potosí, C.P. 79010, Mexico.
Heliyon. 2019 Dec 18;5(12):e03058. doi: 10.1016/j.heliyon.2019.e03058. eCollection 2019 Dec.
The influence of ultrasound-assisted extraction of phytochemicals from Kunth on the antioxidant capacity was investigated. The factors evaluated were: ultrasound extraction time (10, 20 and 30 min), ethanol concentration (0, 35, 70 %) and solid/liquid ratio (1:4, 1:8 and 1:12 g mL). The L (3) array was applied, and the DPPH scavenging capacity of treatments was evaluated to obtain optimal extraction conditions. Finally, the phytochemicals were characterized by high-performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS). Ten minutes of ultrasound extraction using 0 % of ethanol and solid/liquid ratio 1:12 g mL were the optimal conditions of extraction. The HPLC-ESI-MS analysis revealed the presence of gluconic acid, quercetin-3--glucoside, isorhamnetin-3--rutinoside, demethylligstroside, ponicidin, 4-caffeoylquinic acid, rosmarinic acid, and galloyl-hexoside. The optimal ultrasound-assisted extraction conditions were defined by applying the Taguchi methodology. The phytochemicals identified in fruits suggest its use as a potential source of bioactive compounds.
研究了超声辅助提取昆斯植物化学物质对抗氧化能力的影响。评估的因素有：超声提取时间（10、20和30分钟）、乙醇浓度（0、35、70%）和固液比（1:4、1:8和1:12 g/mL）。采用L(3)阵列，并评估处理的DPPH清除能力以获得最佳提取条件。最后，通过高效液相色谱电喷雾电离质谱（HPLC-ESI-MS）对植物化学物质进行表征。使用0%乙醇和固液比1:12 g/mL进行10分钟超声提取是最佳提取条件。HPLC-ESI-MS分析显示存在葡萄糖酸、槲皮素-3-葡萄糖苷、异鼠李素-3-芸香糖苷、去甲基连翘酯苷、ponicidin、4-咖啡酰奎宁酸、迷迭香酸和没食子酰己糖苷。通过应用田口方法确定了最佳超声辅助提取条件。在果实中鉴定出的植物化学物质表明其可作为生物活性化合物的潜在来源。
