Department of Orthodontics, KLES Institute of Dental Sciences, Bangalore, India.
Department of Preventive Dental Science, Division of Orthodontics, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
Am J Orthod Dentofacial Orthop. 2020 Jan;157(1):49-58. doi: 10.1016/j.ajodo.2019.01.026.
The design of the orthodontic bracket or appliance is 1 of the most important factors for creating retentive areas for biofilm formation. In orthodontics, this would be the first study to compare the microbial level changes in 3 different types of orthodontic appliances using checkerboard DNA-DNA hybridization technique. The purpose of this study was to evaluate and compare the extent of appearance of orange and red microbial complexes in patients undergoing orthodontic treatment using aligners, conventional metallic fixed labial appliances, and lingual fixed appliances.
A total of 60 patients, of which 20 patients were undergoing treatment with aligners, 20 patients with labial fixed appliances, and 20 patients with lingual fixed appliances, were included in our study. After 30 days, debonded brackets and rinsed aligners were stored and processed for analysis with checkerboard DNA-DNA hybridization.
Most bacterial species showed moderate counts, with the exception of Treponema denticola, which showed a higher count in all 3 types of appliances. Fusobacterium nucleatum, Porphyromonas gingivalis, and T denticola were present in a higher percentage in the lingual appliance. Fusobacterium periodontium and Prevotella intermedia were present in a higher percentage in the labial fixed appliance. Campylobacter rectus, Tannerella forsythia, and Prevotella melaninogenica counts were moderate in all 3 appliances, with the first 2 microbes showing slightly higher counts in aligners. The association between all the microorganisms were statistically insignificant, with the exception of F nucleatum, which showed a strong statistically significant association in all 3 types of appliances.
The microbial contamination in metallic brackets was higher than that of aligners, when used for a month. Lingual fixed appliances showed more microbial contamination than labial fixed appliances followed by aligners.
正畸托槽或矫治器的设计是为生物膜形成创造固位区的最重要因素之一。在正畸学中，这将是第一项使用斑点杂交 DNA-DNA 技术比较三种不同类型正畸矫治器微生物水平变化的研究。本研究旨在评估和比较使用Aligner、传统金属唇侧固定矫治器和舌侧固定矫治器治疗的正畸患者中橙色和红色微生物复合体出现程度。
本研究共纳入 60 名患者，其中 20 名患者接受Aligner 治疗，20 名患者接受唇侧固定矫治器治疗，20 名患者接受舌侧固定矫治器治疗。30 天后，拆除的托槽和冲洗过的Aligner 被储存并进行斑点杂交 DNA-DNA 分析。
大多数细菌种类的计数呈中度，除了牙龈卟啉单胞菌外，所有三种矫治器的计数均较高。核梭杆菌、牙龈卟啉单胞菌和 T 牙密螺旋体在舌侧矫治器中更为常见。牙周福赛斯坦纳菌和中间普雷沃菌在唇侧固定矫治器中更为常见。直肠弯曲杆菌、 Tannerella forsythia 和 Prevotella melaninogenica 在所有三种矫治器中的计数均为中度，前两种微生物在Aligner 中的计数略高。除核梭杆菌外，所有微生物之间的关联均无统计学意义，核梭杆菌在所有三种矫治器中均表现出强烈的统计学显著关联。
使用一个月后，金属托槽的微生物污染高于Aligner。与唇侧固定矫治器相比，舌侧固定矫治器显示出更多的微生物污染，其次是Aligner。
