Ryavanaki Lingaraj, Tsai Hweiyan, Fuh C Bor
Department of Applied Chemistry, National Chi Nan University, Nantou 545, Taiwan.
Department of Medical Applied Chemistry, Chung Shan Medical University, Taichung 402, Taiwan.
Nanomaterials (Basel). 2020 Jan 2;10(1):94. doi: 10.3390/nano10010094.
Novel bovine serum albumin (BSA)-gold nanoclusters with garlic extract modifications (_G-BSA-AuNCs) were prepared through microwave-assisted rapid synthesis. The modified nanoclusters were characterized and used for the simple and sensitive detection of Pb ions. Both turn-on and turn-off methods were used and compared for Pb ion detection. For Pb ions, the preparation time for the modified nanoclusters was 10 min, and the detection time for the nanoclusters was 6 min. The modified nanoclusters were stable, and their fluorescence intensities changed by less than 10% in 60 days. The detection limit and linear range for the "off-on" method of _G-BSA-AuNCs for Pb ion detection were 0.28 and 1-20 nM, respectively. The recoveries of the _G-BSA-AuNCs probe used for the detection of the Pb(II) ion in tap water ranged from 93.8% to 102.2%, with an average of 97.1%. The "off-on" method of _G-BSA-AuNCs can provide a lower detection limit, higher selectivity, and better recovery than the commonly used "turn-off" methods of _BSA-AuNCs for Pb ion detection. The proposed method is superior to other methods proposed from 2018 to 2019 because it can provide a shorter preparation time and a lower detection limit with good selectivity. The microwave-assisted novel compound, _G-BSA-AuNCs, can be rapidly synthesized in a green manner and can provide a low detection limit, good selectivity, and a simple and fast reaction for Pb ion detection.
通过微波辅助快速合成法制备了具有大蒜提取物修饰的新型牛血清白蛋白（BSA）-金纳米簇（G-BSA-AuNCs）。对修饰后的纳米簇进行了表征，并将其用于铅离子的简单灵敏检测。采用了开启和关闭两种方法并对铅离子检测进行了比较。对于铅离子，修饰后的纳米簇制备时间为10分钟，纳米簇的检测时间为6分钟。修饰后的纳米簇很稳定，其荧光强度在60天内变化小于10%。G-BSA-AuNCs用于铅离子检测的“关闭-开启”方法的检测限和线性范围分别为0.28和1-20 nM。G-BSA-AuNCs探针用于检测自来水中铅（II）离子的回收率在93.8%至102.2%之间，平均为97.1%。与常用的BSA-AuNCs用于铅离子检测的“关闭”方法相比，G-BSA-AuNCs的“关闭-开启”方法可以提供更低的检测限、更高的选择性和更好的回收率。所提出的方法优于2018年至2019年提出的其他方法，因为它可以提供更短的制备时间和更低的检测限，且选择性良好。微波辅助的新型化合物G-BSA-AuNCs可以以绿色方式快速合成，并且可以为铅离子检测提供低检测限、良好的选择性以及简单快速的反应。
