Molecular Enzymology Group, University of Groningen, Nijenborgh 4, Groningen, The Netherlands.
Angew Chem Int Ed Engl. 2020 Mar 16;59(12):4869-4872. doi: 10.1002/anie.201914877. Epub 2020 Feb 4.
Flavoprotein oxidases can catalyze oxidations of alcohols and amines by merely using molecular oxygen as the oxidant, making this class of enzymes appealing for biocatalysis. The FAD-containing (FAD=flavin adenine dinucleotide) alcohol oxidase from P. chrysosporium facilitated double and triple oxidations for a range of aliphatic diols. Interestingly, depending on the diol substrate, these reactions result in formation of either lactones or hydroxy acids. For example, diethylene glycol could be selectively and fully converted into 2-(2-hydroxyethoxy)acetic acid. Such a facile cofactor-independent biocatalytic route towards hydroxy acids opens up new avenues for the preparation of polyester building blocks.
黄素蛋白氧化酶仅能使用分子氧作为氧化剂就能催化醇和胺的氧化，这使得这类酶在生物催化中很有吸引力。黄递酶（FAD=黄素腺嘌呤二核苷酸）来自黄孢原毛平革菌，它能促进一系列脂肪族二醇的双氧化和三氧化。有趣的是，根据二醇底物的不同，这些反应会导致内酯或羟基酸的形成。例如，二甘醇可以选择性地完全转化为 2-(2-羟乙氧基)乙酸。这种方便的、不依赖辅因子的生物催化途径制备羟基酸，为聚酯结构单元的制备开辟了新途径。
