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Nucleotide sequence of the operator-promoter region of the galactose operon of Escherichia coli.

作者信息

Musso R, Di Lauro R, Rosenberg M, de Crombrugghe B

出版信息

Proc Natl Acad Sci U S A. 1977 Jan;74(1):106-10. doi: 10.1073/pnas.74.1.106.

DOI:10.1073/pnas.74.1.106
PMID:319453
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393206/
Abstract

We have derived the nucleotide sequence of a segment of the operator-promoter region of the galactose operon of E. coli, by using a variety of DNA sequencing analyses. We have previously reported the sequence of the 5' terminal portion of gal mRNA [Musso, R. E., de Crombrugghe, B., Pastan, I., Sklar, J., Yot, P. & Weissman, S. (1974) Proc. Natl. Acad. Sci. USA 71, 4940-4944] and of the 59 base pairs preceding the startpoint of gal transcription (J. Sklar, S. Weissman, R. Musso, R. Di Lauro, & B. de Crombrugghe, submitted). In conjunction with those results, the present data provide the sequence of the gal operator-promoter region. This sequence is compared with similar sequences in other promoters and operators. Tentative mechanisms for the regulation of the galactose operon are discussed.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/73b71e4bc496/pnas00023-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/a33652c2f711/pnas00023-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/8f944f4b6625/pnas00023-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/b526d54b3085/pnas00023-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/73b71e4bc496/pnas00023-0115-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/a33652c2f711/pnas00023-0114-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/8f944f4b6625/pnas00023-0114-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/b526d54b3085/pnas00023-0115-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9515/393206/73b71e4bc496/pnas00023-0115-b.jpg

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本文引用的文献

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HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES.大肠杆菌突变体中半乳糖代谢的遗传性缺陷，I. 酶活性的测定
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[REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF THE ENZYMES OF GALACTOSE METABOLISM IN ESCHERICHIA COLI K 12. II. THE GENETIC DETERMINISM OF THE REGULATION].[大肠杆菌K12中半乳糖代谢酶生物合成的调控机制。II. 调控的遗传决定因素]
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[REGULATORY MECHANISMS IN THE BIOSYNTHESIS OF THE ENZYMES OF GALACTOSE METABOLISM IN ESCHERICHIA COLI K 12. I. THE INDUCED BIOSYNTHESIS OF GALACTOKINASE AND THE SIMULTANEOUS INDUCTION OF THE ENZYMATIC SEQUENCE].
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Linkage map of Escherichia coli K-12, edition 6.大肠杆菌K-12连锁图谱，第6版。
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Compilation and analysis of Escherichia coli promoter DNA sequences.大肠杆菌启动子DNA序列的汇编与分析
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Transcription regulatory elements in the late region of bacteriophage T5 DNA.噬菌体T5 DNA晚期区域的转录调控元件。
Nucleic Acids Res. 1983 Nov 11;11(21):7649-58. doi: 10.1093/nar/11.21.7649.
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A control system which causes alternating turn-off/turn-on of transcription on insertion element IS1.一种控制系统，其导致插入元件IS1上的转录交替关闭/开启。
Mol Gen Genet. 1983;192(1-2):61-5. doi: 10.1007/BF00327647.
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Two catabolite activator protein molecules bind to the galactose promoter region of Escherichia coli in the presence of RNA polymerase.在RNA聚合酶存在的情况下，两个分解代谢物激活蛋白分子结合到大肠杆菌的半乳糖启动子区域。
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Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli.galR基因序列表明大肠杆菌中乳糖阻遏蛋白和半乳糖阻遏蛋白有共同的进化起源。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2427-31. doi: 10.1073/pnas.79.8.2427.
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A new type of IS1-mediated deletion.一种新型的由IS1介导的缺失。
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Negative control of the galactose operon in E. coli.
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The 5'-terminal nucleotide sequence of galactose messenger ribonucleic acid of Escherichia coli.大肠杆菌半乳糖信使核糖核酸的5'-末端核苷酸序列。
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4940-4. doi: 10.1073/pnas.71.12.4940.
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The nucleotide sequence of the lac operator.乳糖操纵基因的核苷酸序列。
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