Hall G A, Reynolds D J, Parsons K R, Bland A P, Morgan J H
Institute for Animal Disease Research, Compton Laboratory, Nr Newbury, Berkshire.
Res Vet Sci. 1988 Sep;45(2):240-50. doi: 10.1016/S0034-5288(18)30939-1.
Twenty-one moribund calves with diarrhoea were purchased from 11 farms, their faeces examined for enteropathogens and samples of intestinal tissue removed under anaesthesia. Lesions and presence of enteropathogens on the mucosal surface were scored by histological examination of immunostained paraffin sections. Two or more enteropathogens were detected in 19 calves. Cryptosporidium appeared to be the principal cause of diarrhoea in six calves, rotavirus in four, Salmonella typhimurium in two, bacteria adherent to the surface of the large intestine in two, coronavirus in one and K99+ Escherichia coli in one calf. Diarrhoea in four calves was the consequence of mixed infections in which no one enteropathogen appeared to predominate. In one calf no enteropathogen was detected. Diarrhoea was associated with infections and lesions throughout the small and large intestines. The enteropathogens most frequently associated with lesions in the small intestines were rotavirus, coronavirus and cryptosporidium; in the large intestines they were coronavirus and bacteria apparently adherent to the mucosal surface.
从11个农场购买了21头患有腹泻的濒死犊牛，检查它们的粪便中的肠道病原体，并在麻醉下采集肠道组织样本。通过对免疫染色石蜡切片进行组织学检查，对黏膜表面的病变和肠道病原体的存在情况进行评分。在19头犊牛中检测到两种或更多种肠道病原体。隐孢子虫似乎是6头犊牛腹泻的主要原因，轮状病毒是4头犊牛腹泻的主要原因，鼠伤寒沙门氏菌是2头犊牛腹泻的主要原因，大肠表面附着的细菌是2头犊牛腹泻的主要原因，冠状病毒是1头犊牛腹泻的主要原因，K99 +大肠杆菌是1头犊牛腹泻的主要原因。4头犊牛的腹泻是混合感染的结果，其中没有一种肠道病原体占主导地位。在1头犊牛中未检测到肠道病原体。腹泻与整个小肠和大肠的感染及病变有关。与小肠病变最常相关的肠道病原体是轮状病毒、冠状病毒和隐孢子虫；与大肠病变最常相关的是冠状病毒和明显附着于黏膜表面的细菌。
