Department of Pharmacognosy and Botany, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia.
Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Odbojárov 10, 832 32 Bratislava, Slovakia.
Molecules. 2020 Jan 3;25(1):200. doi: 10.3390/molecules25010200.
Unlike its aerial parts, the underground parts of have so far been studied only marginally. By examining the polyphenolic fingerprint, the antioxidant efficacy and the mutual antioxidant behaviour of mixtures of mint rhizomes, our study presents a modest contribution to addressing this gap. Firstly, we examined the composition of the mint rhizomes: cv. 'Perpeta' (MPP), (ML), and cv. 'Snežna' (MVS). Our LC-MS-DAD analysis revealed the presence of ten compounds belonging to groups of phenolic acids and flavonoids, of which the rosmarinic acid (RA) and lithospermic were most strongly represented. Secondly, we evaluated the antioxidant activity of rhizome infusions by DPPH and ABTS and on NIH/3T3 cell lines by DCFH-DA. Thirdly, we determined, examined, and explained the mutual interactions of rhizome infusions mixtures. While most of the combinations acted additive, synergy was observed in ternary infusion mixtures. The synergic action was also detected in the combination of MPP rhizome infusion and RA in the DCFH-DA test. The combinations of mint rhizomes and rosmarinic acid displayed a high dose-reduction index. This leads to beneficial dose reduction at a given antioxidant effect level in mixtures, compared to the dose of the parts used alone. So far, the pharmaceutical and food industry has not used mint rhizomes in commercial products. Hence, our study draws attention to further applications of the rhizomes as a valuable alternative source of natural antioxidants.
与地上部分不同，迄今为止，地下部分的研究还只是略有涉及。通过检查薄荷根茎的多酚指纹图谱、抗氧化功效以及混合物的相互抗氧化行为，我们的研究为此做出了一定的贡献。首先，我们检查了薄荷根茎的组成：‘Perpeta’（MPP）、普通薄荷（ML）和‘Snežna’（MVS）。我们的 LC-MS-DAD 分析显示，有十种化合物属于酚酸和类黄酮类，其中迷迭香酸（RA）和丹参素的含量最高。其次，我们通过 DPPH 和 ABTS 评估了根茎提取物的抗氧化活性，并通过 DCFH-DA 在 NIH/3T3 细胞系上进行了评估。第三，我们确定、研究并解释了根茎提取物混合物的相互作用。虽然大多数组合表现出相加作用，但在三元混合物中观察到协同作用。在 DCFH-DA 测试中，还观察到 MPP 根茎提取物和 RA 的组合具有协同作用。薄荷根茎和迷迭香酸的组合表现出高剂量降低指数。这使得在混合物中达到给定抗氧化效果水平时，与单独使用部分剂量相比，可以实现有益的剂量降低。迄今为止，制药和食品行业尚未在商业产品中使用薄荷根茎。因此，我们的研究引起了对薄荷根茎进一步应用的关注，将其作为天然抗氧化剂的有价值的替代来源。
