Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, 56000 Cheras, Kuala Lumpur, Malaysia.
School of Nursing, Faculty of Engineering, Science & Technology, Nilai University, 71800 Nilai, Negeri Sembilan, Malaysia.
CNS Neurol Disord Drug Targets. 2020;19(2):115-126. doi: 10.2174/1871527319666200117105133.
Scientific studies support the evidence of the involvement of Metabolic Syndrome (MetS) in the progression of neurodegenerative diseases through oxidative stress. Consumption of antioxidant compounds was found to be beneficial for brain-health as it reduced the brain oxidative stress level and improved cognitive performance in animals. Stingless bee honey or locally known as Kelulut Honey (KH) has high phenolic content and is widely used as a food supplement.
In this study, we aimed to investigate the effects of KH on the brain of MetS-induced rats.
Forty male Wistar rats were divided into 5 groups; 8 weeks (C8) and 16 weeks control groups (C16), groups that received High-Carbohydrate High Fructose (HCHF) diet for 8 weeks (MS8) and 16 weeks (MS16), and a group that received HCHF for 16 weeks with KH supplemented for the last 35 days (KH).
Serum fasting blood glucose decreased in the KH group compared to the MS16 group. HDL levels were significantly decreased in MetS groups compared to control groups. Open field experiments showed that KH group exhibits less anxious behavior compared to the MetS group. Probe trial of Morris water maze demonstrated significant memory retention of KH group compared to the MS16 group. Nissl staining showed a significant decrease in the pyramidal hippocampal cells in the MS16 compared to the KH group.
KH has the ability to normalise blood glucose and reduce serum triglyceride and LDL levels in MetS rats, while behavior studies complement its effect on anxiety and memory. This shows a promising role of KH in attenuating neurodegenerative diseases through the antioxidant activity of its polyphenolic content.
科学研究支持代谢综合征（MetS）通过氧化应激参与神经退行性疾病进展的证据。抗氧化化合物的消耗被发现对大脑健康有益，因为它降低了大脑的氧化应激水平，并改善了动物的认知表现。无刺蜜蜂蜂蜜或当地称为芦菇蜜（KH）具有高酚含量，被广泛用作食品补充剂。
在这项研究中，我们旨在研究 KH 对代谢综合征诱导的大鼠大脑的影响。
将 40 只雄性 Wistar 大鼠分为 5 组; 8 周（C8）和 16 周对照组（C16）、8 周（MS8）和 16 周（MS16）接受高碳水化合物高果糖饮食的组，以及 16 周接受 HCHF 饮食并用 KH 补充最后 35 天的组（KH）。
与 MS16 组相比，KH 组的血清空腹血糖降低。与对照组相比，代谢综合征组的 HDL 水平显著降低。旷场实验表明，KH 组的焦虑行为较代谢综合征组减少。莫里斯水迷宫的探针试验表明，KH 组与 MS16 组相比，记忆保持显著。尼氏染色显示，MS16 组的海马锥体细胞数量明显少于 KH 组。
KH 具有使代谢综合征大鼠的血糖正常化并降低血清甘油三酯和 LDL 水平的能力，而行为研究补充了其对焦虑和记忆的影响。这表明 KH 通过其多酚含量的抗氧化活性在减轻神经退行性疾病方面具有有前景的作用。
