Mercati Francesca, Dall'Aglio Cecilia, Acuti Gabriele, Faeti Valerio, Tardella Federico Maria, Pirino Carolina, De Felice Elena, Scocco Paola
Department of Veterinary Medicine, University of Perugia, Via San Costanzo 4, 06126 Perugia, Italy.
Council for Agricultural Research and Economics, Research Centre for Animal Production and Aquaculture, Modena Unit, Via Beccastecca, 345, 41018 San Cesario sul Panaro, Italy.
Animals (Basel). 2020 Jan 16;10(1):149. doi: 10.3390/ani10010149.
This study evaluated the effects of adding oregano aqueous extract (OAE) to the diet of pig slaughtered at finisher stage. Study was performed to identify glycoconjugates and evaluate the oxidative stress levels in the duodenum and colon intestinal tracts. Glycohistochemistry was performed by staining with Periodic acid-Schiff (PAS), Alcian blue (AB) pH 2.5, AB-PAS, AB pH 1, AB pH 0.5, low iron diamine, and high iron diamine. Serial sections were pre-treated with sialidase V before staining with AB pH 2.5 (Sial-AB) preceded or not by saponification. To study oxidative stress, an immunohistochemical analysis was applied to investigate the presence of the oxidative stress target molecule Bcl-2 Associate X protein (BAX). Findings show that oregano aqueous extract supplementation improves the production of the secretion glycoconjugates involved in direct and indirect defense, thus enhancing the protection of the pig intestinal mucosa. Moreover, the reduced BAX protein immunostaining observed in both duodenum and colon of swine of the oregano-supplemented group respect to that observed in the control group suggests an enhanced antioxidant action by oregano adding. Findings could be useful for other studies aiming to reduce antibiotic use and prevent antimicrobial resistance.
本研究评估了在育肥期屠宰的猪日粮中添加牛至水提取物（OAE）的效果。进行该研究以鉴定糖缀合物，并评估十二指肠和结肠肠道中的氧化应激水平。糖组织化学通过用高碘酸 - 席夫（PAS）、pH 2.5的阿尔辛蓝（AB）、AB - PAS、pH 1的AB、pH 0.5的AB、低铁二胺和高铁二胺染色来进行。在pH 2.5的AB（Sial - AB）染色之前，对连续切片用唾液酸酶V进行预处理，预处理之前或之后进行皂化。为了研究氧化应激，应用免疫组织化学分析来研究氧化应激靶分子Bcl - 2相关X蛋白（BAX）的存在。研究结果表明，补充牛至水提取物可改善参与直接和间接防御的分泌性糖缀合物的产生，从而增强对猪肠黏膜的保护。此外，与对照组相比，在补充牛至的猪的十二指肠和结肠中观察到的BAX蛋白免疫染色减少，表明添加牛至具有增强的抗氧化作用。这些发现可能对其他旨在减少抗生素使用和预防抗菌药物耐药性的研究有用。
