Food and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
Livestock Immunology Unit, International Education and Research Center for Food Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, Japan.
Int J Mol Sci. 2020 Jan 18;21(2):638. doi: 10.3390/ijms21020638.
Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-α. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-α by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-α stimulation, respectively. Expression changes in , , , , , , , , , , , , , , , , , , and genes which are enriched in the 'PPAR signaling' and 'insulin resistance' pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of 'GPCR ligand-binding', 'cell chemotaxis', 'blood coagulation and complement', 'metabolism of lipid and lipoproteins', 'regulation of lipid metabolism by , and 'lipid digestion, mobilization and transport' pathways. TNF-α stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-α to the pMA of intramuscular origin.
脂肪细胞是具有维持身体能量稳态关键功能的动态细胞。脂肪细胞生理学受脂肪生成分化、细胞程序以及生化因子（如血清素和 TNF-α）的外源刺激的影响。在这项工作中，我们研究了猪肌肉前体脂肪细胞（PIP）分化为猪成熟脂肪细胞（pMA）时的全转录组修饰。此外，我们通过微阵列方法研究了 pMA 在用血清素或 TNF-α刺激后的转录组变化。转录组分析显示，分化后或血清素和 TNF-α刺激后，分别有 270、261 和 249 个基因的表达发生了改变。在分化过程中，发现了富集在“PPAR 信号”和“胰岛素抵抗”途径中的 、 、 、 、 、 、 、 、 、 、 、 、 、 、 和 基因的表达变化。血清素刺激剂量依赖性地导致 pMA 中脂肪积累减少。在 pMA 中发现，血清素诱导的差异表达基因参与了“GPCR 配体结合”、“细胞趋化性”、“血液凝固和补体”、“脂质和脂蛋白代谢”、“通过 和 调节脂质代谢”以及“脂质消化、动员和转运”途径的显著富集。TNF-α刺激也导致了与肌内起源的 pMA 中促炎反应相关的转录组修饰。我们的研究结果提供了对脂肪生成和血清素-和 TNF-α对外源刺激的 pMA 的转录组修饰及其相关生物学途径的全景图。
