Truba Joanna, Stanisławska Iwona, Walasek Marta, Wieczorkowska Wioleta, Woliński Konrad, Buchholz Tina, Melzig Matthias F, Czerwińska Monika E
Student Scientific Association, Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, 1 Banacha street, 02-097 Warsaw, Poland.
Department of Bromatology, Medical University of Warsaw, 1 Banacha street, 02-097 Warsaw, Poland.
Plants (Basel). 2020 Jan 18;9(1):122. doi: 10.3390/plants9010122.
The fruits of some species (dogwoods) are used in traditional medicine and considered potential anti-diabetic and hypolipemic agents. The aim of the study was to determine the ability of extracts from (CA), (CF), and (CS) to inhibit digestive enzymes namely -amylase, pancreatic lipase, and -glucosidase, as well as isolation of compounds from plant material with the strongest effect. In addition, the phytochemical profile and antioxidant activity of extracts from three dogwoods were compared with HPLC-DAD-MS/MS and DPPH scavenging assay, respectively. Among the aqueous-ethanolic extracts, the activity of -amylase was the most strongly inhibited by the fruit extract of CA (IC = 115.20 ± 14.31 μg/mL) and the activity of -glucosidase by the fruit of CF (IC = 38.87 ± 2.65 μg/mL). Some constituents of CA fruit extract, such as coumaroylquinic acid, kaempferol, and hydroxytyrosol derivatives, were isolated. Among the three species of dogwood studied, the greatest biological potential was demonstrated by CA extracts, which are sources of phenolic acids and flavonoid compounds. In contrast, iridoid compounds or flavonoid glycosides found in fruits of CF or CS extracts do not play a significant role in inhibiting digestive enzymes but exert antioxidant activity.
某些物种（山茱萸）的果实被用于传统医学，并被认为是潜在的抗糖尿病和降血脂药物。本研究的目的是确定山茱萸（CA）、山茱萸（CF）和山茱萸（CS）提取物抑制消化酶即α-淀粉酶、胰脂肪酶和α-葡萄糖苷酶的能力，以及从具有最强效果的植物材料中分离化合物。此外，分别采用HPLC-DAD-MS/MS和DPPH清除试验比较了三种山茱萸提取物的植物化学特征和抗氧化活性。在水乙醇提取物中，CA果实提取物对α-淀粉酶的活性抑制作用最强（IC = 115.20 ± 14.31 μg/mL），CF果实对α-葡萄糖苷酶的活性抑制作用最强（IC = 38.87 ± 2.65 μg/mL）。分离出了CA果实提取物的一些成分，如香豆酰奎尼酸、山奈酚和羟基酪醇衍生物。在所研究的三种山茱萸中，CA提取物表现出最大的生物潜力，其为酚酸和黄酮类化合物的来源。相比之下，CF或CS提取物果实中发现的环烯醚萜化合物或黄酮苷在抑制消化酶方面不起重要作用，但具有抗氧化活性。
