School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China.
College of pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
Molecules. 2020 Jan 19;25(2):411. doi: 10.3390/molecules25020411.
Three new isoflavone glucosides, kudonol A-C (-), two new ester derivatives of phenylpropanoid, kudolignan A and B (-) and five known compounds, (-)-maackiain (), neoliquiritin (), methyl 4-coumarate (), methyl ferulate () and (+)-wikstromol (), were isolated from an extract of dried seeds of the traditional Chinese medicinal plant L. Their structures were established by NMR and HRESIMS data analyses. The monosaccharide part's configuration of isoflavone glucosides was confirmed by acid hydrolysis and analyzed by a JAsco OR-4090 chiral detector, comparing it to standard substance D-glucose. The cytotoxicity effects against HeLa, Hep3B, MCF-7 and H1299 cells were tested by CCK-8 assay.
从传统中药 L 的干种子提取物中分离得到三种新的异黄酮葡萄糖苷，kudonol A-C（-），两种新的苯丙素酯衍生物，kudolignan A 和 B（-）和五种已知化合物，（-）maackiain（），新甘草苷（），对羟基苯甲酸甲酯（），阿魏酸甲酯（）和（+）wikstromol（）。通过 NMR 和 HRESIMS 数据分析确定了它们的结构。通过酸水解和使用 JAsco OR-4090 手性检测器分析确定异黄酮葡萄糖苷的单糖部分的构型，并与标准物质 D-葡萄糖进行比较。通过 CCK-8 测定法测试了它们对 HeLa、Hep3B、MCF-7 和 H1299 细胞的细胞毒性作用。
