Amanat Shabnam, Taymouri Somayeh, Varshosaz Jaleh, Minaiyan Mohsen, Talebi Ardeshir
Department of Pharmaceutics, School of Pharmacy and Novel Drug Delivery Systems Research Centre, Isfahan University of Medical Sciences, PO Box 81745-359, Isfahan, Iran.
Department of Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran.
Drug Deliv Transl Res. 2020 Oct;10(5):1241-1254. doi: 10.1007/s13346-020-00711-w.
The present study aimed to prepare and investigate the wound healing potential of carboxymethyl cellulose (CMC)-based wafers incorporated with resveratrol (RSV)-loaded cellulose acetate butyrate (CAB) NPs. Accordingly, RSV-CAB NPs were prepared using the solvent evaporation method. The effect of different formulation parameters (polymer content, surfactant concentration, and the volume ratio of aqueous phase to organic phase) on the properties of NPs was investigated using the Box-Behnken design. Then, the optimized NPs were incorporated in wafers comprising CMC combined with hydroxyl propyl methyl cellulose (HPMC) or chitosan. Hydration capacity, porosity, adhesive strength, and hardness of the prepared nanocomposite wafers were examined. Optimized formulation was spherical, showing the particle size, polydispersity index, zeta potential, encapsulation efficiency %, drug loading %, and release efficiency % of 248.5 nm, 0.38, - 1.59, 87.58, 25.94, and 67.10, respectively. The CMC-HPMC wafers exhibited higher porosity, hydration capacity, and adhesive performance, as compared with the CMC wafers alone and CMC-chitosan wafers. Wound healing test and histological evaluation in the excisional wounds of the rats showed that the RSV-NPs-wafers were more effective as a healing accelerator, in comparison to wafers without drug or those containing the free RSV. These results demonstrated the potential of the RSV-NPs-wafer in wound healing drug delivery applications. Graphical abstract.
本研究旨在制备并研究负载白藜芦醇（RSV）的醋酸丁酸纤维素（CAB）纳米粒与羧甲基纤维素（CMC）基薄片的伤口愈合潜力。因此，采用溶剂蒸发法制备了RSV-CAB纳米粒。使用Box-Behnken设计研究了不同配方参数（聚合物含量、表面活性剂浓度以及水相与有机相的体积比）对纳米粒性质的影响。然后，将优化后的纳米粒掺入由CMC与羟丙基甲基纤维素（HPMC）或壳聚糖组成的薄片中。检测了制备的纳米复合薄片的水化能力、孔隙率、粘附强度和硬度。优化后的制剂呈球形，粒径、多分散指数、zeta电位、包封率%、载药率%和释放效率%分别为248.5 nm、0.38、-1.59、87.58、25.94和67.10。与单独的CMC薄片和CMC-壳聚糖薄片相比，CMC-HPMC薄片表现出更高的孔隙率、水化能力和粘附性能。大鼠切除伤口的伤口愈合试验和组织学评估表明，与不含药物的薄片或含有游离RSV的薄片相比，RSV-纳米粒-薄片作为愈合促进剂更有效。这些结果证明了RSV-纳米粒-薄片在伤口愈合药物递送应用中的潜力。图形摘要。
