Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Department of Medicinal Pharmacognosy, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan.
Phytochemistry. 2020 Apr;172:112277. doi: 10.1016/j.phytochem.2020.112277. Epub 2020 Jan 25.
Two undescribed bufadienolide glucosides and four undescribed bufadienolides were isolated from the whole plants of Helleborus foetidus (Ranunculaceae). Their structures were determined by extensive spectroscopic analysis and the results of hydrolytic cleavage. The isolated compounds exhibited cytotoxic activities against HL-60 and A549 cells with IC values ranging from 0.019 to 3.0 μM. The isolated compounds also showed the Na/K ATPase inhibitory activity. The undescribed compound 16β-formyloxy-10,14-dihydroxy-5β-[(β-d-glucopyranosyl)oxy]-19-norbufa-3,20,22-trienolide induced apoptosis in HL-60 cells through a mitochondria-dependent apoptotic pathway.
从嚏根草（毛茛科）全株中分离得到两个未被描述的蟾毒苷葡萄糖苷和四个未被描述的蟾毒苷。通过广泛的光谱分析和水解裂解的结果确定了它们的结构。分离得到的化合物对 HL-60 和 A549 细胞表现出细胞毒性，IC 值范围为 0.019 至 3.0 μM。这些分离得到的化合物还表现出 Na/K ATP 酶抑制活性。未被描述的化合物 16β-甲酰氧基-10,14-二羟基-5β-[(β-D-吡喃葡萄糖基)氧基]-19-去甲福马烷-3,20,22-三烯醇内酯通过线粒体依赖性凋亡途径诱导 HL-60 细胞凋亡。
