Department for the Innovation in Biological, Agrofood and Forestal Systems, Tuscia University, 01100 Viterbo, Italy.
High Equipment Centre, Tuscia University, 01100 Viterbo, Italy.
Molecules. 2020 Jan 26;25(3):538. doi: 10.3390/molecules25030538.
Recent scientific investigations have reported a number of essential oils to interfere with intracellular signalling pathways and to induce apoptosis in different cancer cell types. In this paper, Lavandin Essential Oil (LEO), a natural sterile hybrid obtained by cross-breeding × was tested on human leukaemia cells (HL60). Based on the MTT results, the reduced cell viability of HL60 cells was further investigated to determine whether cell death was related to the apoptotic process. HL60 cells treated for 24 h with LEO were processed by flow cytometry, and the presence of Annexin V was measured. The activation of caspases-3 was evaluated by western blot and immunofluorescence techniques. Treated cells were also examined by scanning and transmission electron microscopy to establish the possible occurrence of morphological alterations during the apoptotic process. LEO main compounds, such as linalool, linalyl acetate, 1,8-cineole, and terpinen-4-ol, were also investigated by MTT and flow cytometry analysis. The set of obtained results showed that LEO treatments induced apoptosis in a dose-dependent, but not time-dependent, manner on HL60 cells, while among LEO main compounds, both terpinen-4-ol and linalyl acetate were able to induce apoptosis.
最近的科学研究报告称，一些精油可以干扰细胞内信号通路，并诱导不同类型的癌细胞凋亡。本文以薰衣草精油（LEO）为研究对象，LEO 是一种通过杂交 × 获得的天然无菌杂种，对人白血病细胞（HL60）进行了测试。基于 MTT 结果，进一步研究了 HL60 细胞活力降低是否与细胞凋亡过程有关。用 LEO 处理 HL60 细胞 24 小时后，通过流式细胞术处理，并测量 Annexin V 的存在。通过 Western blot 和免疫荧光技术评估半胱天冬酶-3 的激活。通过扫描和透射电子显微镜检查处理过的细胞，以确定在凋亡过程中是否可能发生形态改变。还通过 MTT 和流式细胞术分析研究了 LEO 的主要化合物，如芳樟醇、乙酸芳樟酯、1,8-桉叶油醇和萜品-4-醇。获得的结果表明，LEO 处理以剂量依赖但非时间依赖的方式诱导 HL60 细胞凋亡，而在 LEO 的主要化合物中，萜品-4-醇和乙酸芳樟酯都能够诱导细胞凋亡。
