Patil Shriniwas P
Department of Pharmacognosy, SCES's Indira College of Pharmacy, Pune, 411 038, India.
Heliyon. 2020 Jan 22;6(1):e03171. doi: 10.1016/j.heliyon.2020.e03171. eCollection 2020 Jan.
(Scrophulariaceae/Orobanchaceae) is a rarely occurring parasitic plant grows on roots of L. (Verbenaceae). As per Indian system of medicine, Ayurveda, it can be used in treatment of various diseases. So far, this plant has not been explored phyto-chemically in detail. Non-small cell lung cancer (NSCLC) is mostly occurring type of lung cancer, which so far can be treated by chemotherapy approach including cisplatin.
Present research work was aimed towards preparation of methanolic fraction of rhizomes; its phytochemical analysis by 1H-NMR and HR-LC/MS; evaluation of its anti-cancer property against NSCLC-A549 cell line.
For preparation of methanolic fraction (MF), rhizome powder was defatted; extracted with combination of water and alcohol (1:1); added with lead acetate and then sulphuric acid; fractionation of ethyl acetate fraction with methanol. After phytochemical analysis of MF by preliminary chemical testing, TLC, H-NMR and HR-LC/MS techniques, MF was screened for its anti - cancer property against NSCLC-A549 cell line by MTT assay.
Detail phytochemical analysis reflected successful preparation of tannin-less MF of rhizomes. Different types of analytical techniques first time proved the proved the presence of various types of phytochemicals in this plant. On MTT assay, it was found that MF has anti-cancer property against NSCLC-A549 cell line with IC value, 306.51 μg/ml.
MF contains different phytochemicals like iridoids, flavonoids, steroid glycosides and also strigalactones; which cumulatively exert anti-cancer effect on A549. Appearance of all these compounds is significant in chemotaxonomic surveillance of this rare plant and specially, strigalactones can be proved important in establishing their parasitism with host plant.
（玄参科/列当科）是一种罕见的寄生植物，生长在（马鞭草科）植物的根部。根据印度传统医学阿育吠陀，它可用于治疗多种疾病。到目前为止，尚未对该植物进行详细的植物化学研究。非小细胞肺癌（NSCLC）是最常见的肺癌类型，目前可通过包括顺铂在内的化疗方法进行治疗。
目前的研究工作旨在制备根茎的甲醇提取物；通过1H-NMR和HR-LC/MS对其进行植物化学分析；评估其对NSCLC-A549细胞系的抗癌特性。
为制备甲醇提取物（MF），将根茎粉末脱脂；用水和乙醇（1:1）混合提取；加入醋酸铅，然后加入硫酸；用甲醇对乙酸乙酯提取物进行分馏。通过初步化学测试、TLC、H-NMR和HR-LC/MS技术对MF进行植物化学分析后，通过MTT法筛选MF对NSCLC-A549细胞系的抗癌特性。
详细的植物化学分析表明成功制备了无单宁的根茎甲醇提取物。不同类型的分析技术首次证明了该植物中存在各种类型的植物化学物质。通过MTT法发现，MF对NSCLC-A549细胞系具有抗癌特性，IC值为306.51μg/ml。
MF含有不同的植物化学物质，如环烯醚萜、黄酮类、甾体糖苷以及独脚金内酯；这些物质共同对A549发挥抗癌作用。所有这些化合物的出现对于这种稀有植物的化学分类监测具有重要意义，特别是独脚金内酯在证明其与寄主植物的寄生关系方面可能很重要。
