Shen Yangyang, Zhang Jianli, Gui Hongbing, Wang Huili, Li Yinxia, Zhang Jun, Cao Shaoxian, Zhong Jifeng, Qian Yong, Meng Chunhua
Institute of Animal Science, Jiangsu Academy of Agriculture Sciences, Nanjing 210014, China.
Key Laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China.
Metabolites. 2023 Dec 17;13(12):1201. doi: 10.3390/metabo13121201.
Garlic, an important economic crop, provides nutrient-rich straw. When appropriately balanced with silage corn stalks, it is a high-quality forage resource. However, studies on the impact of garlic straw with silage corn stalks on Hu sheep's digestive metabolism and rumen microbiota are scarce. In this study, different addition ratios of garlic straw and silage corn stalks were utilized for in vitro experiments. We designed six experimental groups (CON, G0, G20, G40, G60, G80, and G100) based on varying ratios of garlic straw to silage corn stalks. Rumen microbiota was analyzed through 16S rRNA sequencing. Nutrient composition analysis indicated that garlic straw's relative feeding value (RFV) closely resembled that of silage corn stalks. After 24 h of fermentation, dry matter digestibility and in vitro gas production significantly increased, reaching peak values at a 60% addition ratio. Furthermore, volatile fatty acids (VFAs) such as acetic, propionic, and butyric acid exhibited elevated contents, with the highest yields observed at 60% inclusion. At the genus level, , gut group, and were identified as the dominant bacterial groups. The gas production test showed a significant decrease in the G80 group compared to others. Microbial analysis revealed a higher abundance of in G80 compared to G20, offering valuable insights for reducing greenhouse gas emissions from ruminant animals. Finally, this study predicted the impact of garlic straw with silage corn stalks' addition on Hu sheep's metabolic pathways and biological functions of the rumen microbiota. This research highlights the potential for effectively utilizing garlic straw as a feed resource for Hu sheep and proposes a rational proportion for combining garlic straw with silage corn stalks.
大蒜是一种重要的经济作物，其秸秆营养丰富。与青贮玉米秸秆适当搭配时，它是一种优质的饲料资源。然而，关于大蒜秸秆与青贮玉米秸秆对湖羊消化代谢和瘤胃微生物群影响的研究较少。在本研究中，采用了不同添加比例的大蒜秸秆和青贮玉米秸秆进行体外实验。我们根据大蒜秸秆与青贮玉米秸秆的不同比例设计了六个实验组（CON、G0、G20、G40、G60、G80和G100）。通过16S rRNA测序分析瘤胃微生物群。营养成分分析表明，大蒜秸秆的相对饲养价值（RFV）与青贮玉米秸秆相近。发酵24小时后，干物质消化率和体外产气量显著增加，在添加比例为60%时达到峰值。此外，乙酸、丙酸和丁酸等挥发性脂肪酸（VFA）含量升高，在添加量为60%时产量最高。在属水平上， 、肠道菌群和 被确定为主要细菌群。产气试验表明，G80组与其他组相比产气显著降低。微生物分析显示，G80组中的 丰度高于G20组，这为减少反刍动物的温室气体排放提供了有价值的见解。最后，本研究预测了添加大蒜秸秆与青贮玉米秸秆对湖羊代谢途径和瘤胃微生物群生物学功能的影响。这项研究突出了有效利用大蒜秸秆作为湖羊饲料资源的潜力，并提出了大蒜秸秆与青贮玉米秸秆合理的混合比例。
