Department of Sports Medicine, Department of Orthopedics, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai 200233, People's Republic of China.
Scientific Research Department of Shanghai University of Engineering Science, Shanghai 201620, People's Republic of China.
Int J Nanomedicine. 2019 Nov 13;14:8891-8903. doi: 10.2147/IJN.S210329. eCollection 2019.
In this study, we attempted to regulate the preparation of Alg-CS-Flu three-dimensional scaffolds via a facile freeze-drying method combined with amidation.
Three-dimensional porous flurbiprofen-grafted alginate (Alg)-chitosan (CS) scaffolds were successfully prepared by a facile freeze-drying method combined with amidation for skin tissue engineering applications. Alg-CS composite was first used to load flurbiprofen (Flu), which is a kind of anti-inflammatory non-steroidal molecule. The Flu-loaded Alg/CS composite solution, through freeze-drying and 1-ethyl-3(3-(dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide crosslinking to form an Alg-CS-Flu scaffold, exhibited a uniform and porous morphology that was characterized using scanning electron microscopy. The Alg-CS-Flu as-prepared scaffold was also characterized using Fourier-transform infrared spectroscopy, water contact angle, thermal properties, and stress-strain testing.
The results reveal that Flu was successfully grafted onto the surfaces of the Alg-CS-Flu scaffold, which showed good hydrophilicity and appropriate mechanical properties. Furthermore, cell viability, cell morphology from cells cultured in vitro, and hematoxylin-eosin staining after the graft was subcutaneously embedded in mice for 7 d demonstrated that the Alg-CS-Flu scaffold had no unfavorable effects on the adhesion and proliferation of fibroblasts, as well as a good anti-inflammatory property.
The developed Alg-CS-Flu scaffold is proposed as a promising material or skin tissue engineering application.
在这项研究中，我们试图通过一种简便的冷冻干燥方法结合酰胺化来调节 Alg-CS-Flu 三维支架的制备。
为了应用于皮肤组织工程，我们成功地通过一种简便的冷冻干燥方法结合酰胺化制备了具有多孔结构的载有氟比洛芬（一种抗炎非甾体分子）的海藻酸钠（Alg）-壳聚糖（CS）三维支架。Alg-CS 复合材料首先用于负载氟比洛芬（Flu）。通过冷冻干燥和 1-乙基-3（3-（二甲基氨基）丙基）碳二亚胺/N-羟基琥珀酰亚胺交联形成 Alg-CS-Flu 支架，对载有 Flu 的 Alg/CS 复合溶液进行处理，得到的 Alg-CS-Flu 支架具有均匀的多孔形貌，这一特征可以通过扫描电子显微镜观察到。我们还通过傅里叶变换红外光谱、水接触角、热性能和应力-应变测试对 Alg-CS-Flu 支架进行了表征。
结果表明，Flu 成功地接枝到 Alg-CS-Flu 支架的表面，该支架具有良好的亲水性和适当的机械性能。此外，体外培养的细胞的细胞活力、细胞形态和皮下植入小鼠 7 天后的苏木精-伊红染色结果表明，Alg-CS-Flu 支架对成纤维细胞的黏附和增殖没有不利影响，并且具有良好的抗炎性能。
所开发的 Alg-CS-Flu 支架有望成为一种有前途的皮肤组织工程应用材料。
