对脱氧核糖核酸有亲和力的大肠杆菌膜蛋白。
Escherichia coli membrane proteins with an affinity for deoxyribonucleic acid.
作者信息
Kohiyama M, Kollek R, Goebel W, Kepes A
出版信息
J Bacteriol. 1977 Feb;129(2):658-67. doi: 10.1128/jb.129.2.658-667.1977.
Abstract
From the membrane fraction of Escherichia coli K-12 strain, four protein fractions (peaks I, IIa, IIb, and III) which have affinity for deoxyribonucleic acid (DNA) have been isolated. The molecular weights of these proteins are between 12,000 and 8,000. Only the peak III fraction contains a protein that binds preferentially to single-stranded DNA, whereas the others contain proteins that bind also to double-stranded DNA. The binding activity of the peak IIb protein is inhibited in the presence of polyuridylic acid. Peak I and peak IIa protein fractions behave like hydrophobic proteins.
摘要
从大肠杆菌K - 12菌株的膜组分中，已分离出四种对脱氧核糖核酸（DNA）具有亲和力的蛋白质组分（峰I、IIa、IIb和III）。这些蛋白质的分子量在12,000至8,000之间。只有峰III组分含有一种优先与单链DNA结合的蛋白质，而其他组分则含有也能与双链DNA结合的蛋白质。在多聚尿苷酸存在的情况下，峰IIb蛋白质的结合活性受到抑制。峰I和峰IIa蛋白质组分表现得像疏水蛋白质。
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