Department of Food and Nutrition, KC University, Seoul 07661, Korea.
Department of Food Science and Technology, Seoul National University of Science and Technology, Seoul 01811, Korea.
Nutrients. 2020 Feb 5;12(2):411. doi: 10.3390/nu12020411.
Atopic dermatitis (AD) is a chronic inflammatory skin disease caused mainly by immune dysregulation. This study explored the anti-inflammatory and immunomodulatory effects of the ethanol extract (CA) on an AD-like dermal disorder. Treatment with CA inhibited the expression of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in a dose-dependent manner in inflammatory stimulated HaCaT cells by interferon-γ (IFN-γ) and TNF-α-triggered inflammation. Eight-week-old BALB/c mice treated with 2,4-dinitrochlorobenzene (DNCB) were used as a mouse model of AD. In AD induce model, we had two types treatment of CA; skin local administration (80 µg/cm, AD+CA-80) and oral administration (200 mg/kg/d, AD+CA-200). Interestingly, the CA-treated groups exhibited considerably decreased mast cell infiltration in the ear tissue. In addition, the expression of IL-6 in mast cells, as well as the expression of various pathogenic cytokines, such as TNF-α, IL-4, IL-5, IL-6, IL-10, IL-17, iNOS, COX-2, and CXCL9, was reduced in both AD+CA-80 and AD+CA-200 groups. Collectively, our data demonstrate the pharmacological role and signaling mechanism of CA in the regulation of allergic inflammation of the skin, which supports our hypothesis that CA could potentially be developed as a therapeutic agent for AD.
特应性皮炎（AD）是一种主要由免疫失调引起的慢性炎症性皮肤病。本研究探讨了 乙醇提取物（CA）对类似 AD 的皮肤疾病的抗炎和免疫调节作用。CA 以剂量依赖的方式抑制干扰素-γ（IFN-γ）和 TNF-α 触发炎症刺激的 HaCaT 细胞中白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达。用 2,4-二硝基氯苯（DNCB）处理的 8 周龄 BALB/c 小鼠被用作 AD 的小鼠模型。在 AD 诱导模型中，我们用两种 CA 处理方法；皮肤局部给药（80 µg/cm，AD+CA-80）和口服给药（200 mg/kg/d，AD+CA-200）。有趣的是，CA 处理组的耳部组织中肥大细胞浸润明显减少。此外，AD+CA-80 和 AD+CA-200 组中肥大细胞中 IL-6 的表达以及各种致病细胞因子（如 TNF-α、IL-4、IL-5、IL-6、IL-10、IL-17、iNOS、COX-2 和 CXCL9）的表达均降低。总之，我们的数据表明 CA 在调节皮肤过敏炎症方面具有药理作用和信号机制，这支持了我们的假设，即 CA 有可能被开发为 AD 的治疗药物。
