Department of Sports and Health, Guangzhou Sport University, Guangzhou 510500, China.
Institute for Health and Sport, Victoria University, Melbourne 8001, Australia.
Biomed Res Int. 2020 Jan 23;2020:3030793. doi: 10.1155/2020/3030793. eCollection 2020.
To identify noninvasive immune biomarkers of exercise-induced immunosuppression using the iTRAQ proteomics technique.
Fifteen healthy males were recruited and subjected to a four-week incremental treadmill running training program. After each week of training, WBC counts and CD4 and CD8 lymphocytes were measured to monitor the immune function status. iTRAQ proteomics technology was used to identify differential proteins and their characteristics in urine.
Our data showed that the WBC counts, CD4 lymphocytes, and CD4/CD8 ratio decreased by more than 10% after four weeks of training, suggesting exercise-induced immunosuppression. A total of 1854 proteins were identified in urine during the incremental running using the iTRAQ technology. Compared with the urine before training, there were 89, 52, 77, and 148 proteins significantly upregulated and 66, 27, 68, and 114 proteins significantly downregulated after each week, respectively. Among them, four upregulated proteins, SEMG-1, PIP, PDGFRL, and NDPK, increased their abundance with the increased exercise intensity. Bioinformatics analysis indicates that these proteins are involved in stress response and immune function.
Four weeks of incremental treadmill running induced immunosuppression in healthy males. By using iTRAQ proteomics, four proteins in the urine, SEMG-1, PIP, PDGFRL, and NDPK, were found to increase incrementally with the increased exercise intensity, which have the potential to be used as noninvasive immune biomarkers of exercise-induced immunosuppression.
使用 iTRAQ 蛋白质组学技术鉴定运动诱导免疫抑制的非侵入性免疫生物标志物。
招募了 15 名健康男性，并进行了为期四周的递增跑步机跑步训练计划。在每周的训练后，测量白细胞计数和 CD4 和 CD8 淋巴细胞，以监测免疫功能状态。使用 iTRAQ 蛋白质组学技术鉴定尿液中的差异蛋白及其特征。
我们的数据显示，四周的训练后白细胞计数、CD4 淋巴细胞和 CD4/CD8 比值下降了 10%以上，表明运动诱导了免疫抑制。使用 iTRAQ 技术在递增跑步过程中鉴定出了 1854 种尿液蛋白。与训练前的尿液相比，分别有 89、52、77 和 148 种蛋白在上周分别显著上调，66、27、68 和 114 种蛋白显著下调。其中，四个上调的蛋白 SEMG-1、PIP、PDGFRL 和 NDPK，其丰度随运动强度的增加而增加。生物信息学分析表明，这些蛋白参与应激反应和免疫功能。
四周的递增跑步机跑步导致健康男性的免疫抑制。通过使用 iTRAQ 蛋白质组学，发现尿液中的四个蛋白 SEMG-1、PIP、PDGFRL 和 NDPK 随运动强度的增加而递增，具有作为运动诱导免疫抑制的非侵入性免疫生物标志物的潜力。
