Liao Cangsong, Seebeck Florian P
Department for Chemistry, University of Basel, Mattenstrasse 24a, BPR 1002, 4056, Basel, Switzerland.
Angew Chem Int Ed Engl. 2020 Apr 27;59(18):7184-7187. doi: 10.1002/anie.201916025. Epub 2020 Mar 11.
This report describes a modular enzyme-catalyzed cascade reaction that transforms l- or d-α-amino acids to β-methyl-α-amino acids. In this process an α-amino acid transaminase, an α-keto acid methyltransferase, and a halide methyltransferase cooperate in two orthogonal reaction cycles that mediate product formation and regeneration of the cofactor pyridoxal-5'-phosphate and the co-substrate S-adenosylmethionine. The only stoichiometric reagents consumed in this process are the unprotected l- or d-α-amino acid and methyl iodide.
本报告描述了一种模块化酶催化级联反应，该反应可将l-或d-α-氨基酸转化为β-甲基-α-氨基酸。在此过程中，一种α-氨基酸转氨酶、一种α-酮酸甲基转移酶和一种卤化物甲基转移酶在两个正交反应循环中协同作用，介导辅因子磷酸吡哆醛-5'-磷酸和共底物S-腺苷甲硫氨酸的产物形成和再生。该过程中消耗的唯一化学计量试剂是未保护的l-或d-α-氨基酸和碘甲烷。
