Department of Paleobiotechnology, Leibniz Institute for Natural Product Research and Infection Biology Hans Knöll Institute (HKI), Beutenbergstraße 11a, 07745 Jena, Germany.
Org Biomol Chem. 2020 Mar 4;18(9):1710-1727. doi: 10.1039/c9ob02539a.
Nonribosomal lipopeptides (NRLPs) are complex natural products of bacterial origin that not only fulfill important ecological functions but also serve as lead structures for the development of new pharmaceuticals. In order to carry out detailed structure-activity relationship studies and to decipher the biological activities of NRLPs, the primary structure, including stereochemical assignment, of every new member of this natural product family has to be established first. In this review, we want to focus on analytical techniques and tools that can be employed to elucidate the structure of bacterial NRLPs.
非核糖体脂肽（NRLP）是细菌来源的复杂天然产物，不仅具有重要的生态功能，而且还可作为开发新药物的先导结构。为了进行详细的结构-活性关系研究并阐明 NRLP 的生物活性，必须首先确定该天然产物家族的每个新成员的一级结构，包括立体化学分配。在这篇综述中，我们希望重点介绍可用于阐明细菌 NRLP 结构的分析技术和工具。
