一项与昼夜节律相关的 MTNR1B 基因变异（rs10830963）可调节 9 个月高蛋白/低碳水化合物饮食与标准低热量饮食后体重变化和胰岛素抵抗。
A circadian rhythm-related MTNR1B genetic variant (rs10830963) modulate body weight change and insulin resistance after 9 months of a high protein/low carbohydrate vs a standard hypocaloric diet.
机构信息
Endocrinology and Nutrition Research Center, School of Medicine, Department of Endocrinology and Nutrition, Hospital Clinico Universitario, University of Valladolid, Valladolid, Spain.
Endocrinology and Nutrition Research Center, School of Medicine, Department of Endocrinology and Nutrition, Hospital Clinico Universitario, University of Valladolid, Valladolid, Spain.
出版信息
J Diabetes Complications. 2020 Apr;34(4):107534. doi: 10.1016/j.jdiacomp.2020.107534. Epub 2020 Jan 13.
BACKGROUND & AIMS: The risk allele (G) of rs10830963 in the melatonin receptor 1 B (MTNR1B) gene presents an association with biochemical parameters and obesity. We study the effect of this SNP on insulin resistance and weight loss secondary to two hypocaloric diets.
METHODS
270 obese subjects were randomly allocated during 9 months (Diet HP: a high protein/low carbohydrate vs. Diet S: a standard severe hypocaloric diets). Anthropometric parameters, fasting blood glucose, C-reactive protein (CRP), insulin concentration, insulin resistance (HOMA-IR), lipid profile and adipocytokines levels were measured. Genotype of MTNR1B gene polymorphism (rs10830963) was evaluated.
RESULTS
All adiposity parameters, systolic blood pressure and leptin levels decreased in all subjects after both diets. This improvement of adiposity parameters was higher in non-G allele carriers than G allele carriers. After weight loss with Diet HP, (CC vs. CG + GG at 9 months); total cholesterol (delta: -9.9 ± 2.4 mg/dl vs. -4.8 ± 2.2 mg/dl:p < 0.05), LDL-cholesterol (delta: -8.3 ± 1.9 mg/dl vs. -5.1 ± 2.2 mg/dl: p < 0.05), insulin (delta: -4.7 ± 0.8 UI/L vs. -0.9 ± 1.0 UI/L: p < 0.05), triglycerides (delta: -17.7 ± 3.9 mg/dl vs. -6.1 ± 2.8 mg/dl: p < 0.05) and HOMA IR (delta: -0.8 ± 0.2 units vs. -0.2 ± 0.1 units: p < 0.05) improved only in no G allele carriers. After weight loss with Diet S in non G allele carriers, insulin levels (delta (CC vs. CG + GG): -3.4 ± 0.6 UI/L vs. -1.2 ± 0.4 UI/L: p < 0.05), triglycerides (delta: -29.2 ± 3.4 mg/dl vs. -8.2 ± 3.8 mg/dl: p < 0.05), HOMA-IR (delta (CC vs. CG + GG): -1.1 ± 0.2 units vs. -0.1 ± 0.1 units: p < 0.05), total cholesterol (delta: -15.9 ± 7.4 mg/dl vs. -5.8 ± 2.9 mg/dl:ns) and LDL-cholesterol (delta: -13.7 ± 5.9 mg/dl vs. -6.0 ± 2.9 mg/dl: ns) decreased, too.
CONCLUSIONS
our study detected a relationship of rs10830963 variant of MTNR1B gene with adiposity changes, cholesterol changes and insulin resistance modification induced by two different hypocaloric during 9 months.
背景与目的
褪黑素受体 1 B（MTNR1B）基因中的 rs10830963 风险等位基因（G）与生化参数和肥胖有关。我们研究了该 SNP 对两种低热量饮食引起的胰岛素抵抗和体重减轻的影响。
方法
270 名肥胖受试者在 9 个月内随机分配（饮食 HP：高蛋白/低碳水化合物与饮食 S：标准严重低热量饮食）。测量了人体测量参数、空腹血糖、C 反应蛋白（CRP）、胰岛素浓度、胰岛素抵抗（HOMA-IR）、血脂谱和脂肪细胞因子水平。评估了 MTNR1B 基因多态性（rs10830963）的基因型。
结果
所有受试者在两种饮食后，所有肥胖参数、收缩压和瘦素水平均降低。非 G 等位基因携带者的这种肥胖参数改善程度高于 G 等位基因携带者。在 HP 饮食减肥后（9 个月时 CC 与 CG+GG 相比）；总胆固醇（差值：-9.9±2.4mg/dl 与-4.8±2.2mg/dl：p<0.05）、LDL 胆固醇（差值：-8.3±1.9mg/dl 与-5.1±2.2mg/dl：p<0.05）、胰岛素（差值：-4.7±0.8UI/L 与-0.9±1.0UI/L：p<0.05）、甘油三酯（差值：-17.7±3.9mg/dl 与-6.1±2.8mg/dl：p<0.05）和 HOMA-IR（差值：-0.8±0.2 单位与-0.2±0.1 单位：p<0.05）仅在非 G 等位基因携带者中改善。在 S 饮食减肥后，非 G 等位基因携带者的胰岛素水平（CC 与 CG+GG 相比：-3.4±0.6UI/L 与-1.2±0.4UI/L：p<0.05）、甘油三酯（差值：-29.2±3.4mg/dl 与-8.2±3.8mg/dl：p<0.05）、HOMA-IR（CC 与 CG+GG 相比：-1.1±0.2 单位与-0.1±0.1 单位：p<0.05）、总胆固醇（差值：-15.9±7.4mg/dl 与-5.8±2.9mg/dl：ns）和 LDL 胆固醇（差值：-13.7±5.9mg/dl 与-6.0±2.9mg/dl：ns）也有所下降。
结论
我们的研究检测到 MTNR1B 基因 rs10830963 变体与 9 个月内两种不同低热量饮食引起的肥胖变化、胆固醇变化和胰岛素抵抗修饰之间的关系。
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