Wahyuni Dwi Kusuma, Purnobasuki Hery, Kuncoro Eko Prasetyo, Ekasari Wiwied
Department of Biology, Faculty of Science and Technology, Airlangga University Surabaya, East Java, 60115, Indonesia.
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Airlangga University Surabaya, East Java, 60115, Indonesia.
Afr J Infect Dis. 2020 Jan 8;14(1):1-7. doi: 10.21010/ajid.v14i1.1. eCollection 2020.
Malaria is a global health problem that requires urgent need for new drugs. Tempuyung ( L.) possesses many potential medicinal compounds. As the plant is originally found wild, it is important to reproduce its secondary metabolites by tissue culture. The objectives of this study were to look for effective methods to induce callus from leaf explants of L. and to test its antiplasmodial activity.
The leaves and petioles of the plant were cultured on Murashige and Skoog (MS) solid medium supplemented with indole acetic-3-acid (IAA), indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), 2,4-dichlorophenoxyacetic acid (2,4-D) and benzyl amino purine (BAP), in light and dark incubations. The best results obtained from callus induction were then treated by with several concentrations of sucrose (1- 5%). The best results from callus induction were then extracted with methanol for antiplasmodial test by Trager and Jensen's method. It was also tested against 3D7 strain of .
The combination of 1mg/L 2,4-D and 0.5 mg/L BAP in dark incubation was the best treatment for callus induction of tempuyung. It produced the best quality of callus and the shortest period for callusing. Sucrose treatment had various effects on leaves callusing, but had no effect on petioles callusing, whereby 4% sucrose was the best treatment for leaves callusing in dark incubation. The methanol extract of the best callus had anti-plasmodial activity with IC=0.343 µg/mL.
Methanol extract of tempuyung callus shows potential as an antimalarial drug but more studies would be required.
疟疾是一个全球性的健康问题，迫切需要新的药物。Tempuyung（L.）含有许多潜在的药用化合物。由于该植物最初是在野外发现的，通过组织培养来繁殖其次生代谢产物很重要。本研究的目的是寻找从Tempuyung的叶片外植体诱导愈伤组织的有效方法，并测试其抗疟活性。
将该植物的叶片和叶柄在添加了吲哚乙酸-3-酸（IAA）、吲哚-3-丁酸（IBA）、萘乙酸（NAA）、2,4-二氯苯氧乙酸（2,4-D）和苄基氨基嘌呤（BAP）的Murashige和Skoog（MS）固体培养基上进行培养，分别置于光照和黑暗条件下。从愈伤组织诱导获得的最佳结果然后用几种浓度的蔗糖（1 - 5%）处理。然后用甲醇提取愈伤组织诱导的最佳结果用于通过Trager和Jensen方法进行抗疟试验。它还针对疟原虫的3D7菌株进行了测试。
在黑暗培养条件下，1mg/L 2,4-D和0.5mg/L BAP的组合是诱导Tempuyung愈伤组织的最佳处理方法。它产生了质量最好的愈伤组织和最短的愈伤形成时间。蔗糖处理对叶片愈伤形成有不同影响，但对叶柄愈伤形成没有影响，其中4%蔗糖是黑暗培养条件下叶片愈伤形成的最佳处理。最佳愈伤组织的甲醇提取物具有抗疟活性，IC50 = 0.343μg/mL。
Tempuyung愈伤组织的甲醇提取物显示出作为抗疟药物的潜力，但还需要更多的研究。
