Santos Nathally C de S, Scodro Regiane B de L, Leal Dayane Cb, do Prado Silvia Mt, Micheletti Daniela F, Sampiron Eloísa G, Costacurta Giovana F, de Almeida Aryadne L, da Silva Liliani Af, Ieque Andressa L, Ghiraldi Lopes Luciana D, de Pádua Rubia Af, Siqueira Vera Ld, Caleffi-Ferracioli Katiany R, Cardoso Rosilene F
Postgraduate Program in Bioscience and Physiopathology, State University of Maringá, Paraná, Brazil.
Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
Future Microbiol. 2020 Jan;15:107-114. doi: 10.2217/fmb-2019-0050. Epub 2020 Feb 17.
To evaluate an assay to detect minimum bactericidal concentration (MBC) in , using as single model rifampicin, isoniazid, levofloxacin (LVX) and linezolid (LNZ) and in combination. MBCs were carried out directly from resazurin microtiter assay plate and 3D checkerboard in HRv and five resistant clinical isolates. The proposed MBC assay showed similar values to those determined by MGIT™, used as control. LVX and LNZ's MBC values were close to their MIC values. LNZ or LVX combined with isoniazid and rifampicin showed MBC value reduced in 63.7% of the assays. The proposed assay to determine MBCs of drugs can be applied to the study of new compounds with anti- activity to detect their bactericidal effect and also in laboratory routine for clinical dose adjustment of drugs according to the patient's profile.
为评估一种检测最低杀菌浓度（MBC）的方法，以利福平、异烟肼、左氧氟沙星（LVX）和利奈唑胺（LNZ）作为单一模型及联合用药模型，在人型结核分枝杆菌（HRv）和五株耐药临床分离株中进行研究。MBC测定直接在刃天青微量滴定板和三维棋盘法中对HRv和五株耐药临床分离株进行。所提出的MBC检测方法显示的值与用作对照的MGIT™测定法所确定的值相似。LVX和LNZ的MBC值接近其MIC值。LNZ或LVX与异烟肼和利福平联合使用时，在63.7%的检测中MBC值降低。所提出的测定药物MBC的方法可应用于研究具有抗活性的新化合物以检测其杀菌效果，也可用于实验室常规操作，根据患者情况调整药物临床剂量。
