Department of Periodontology, Manipal College of Dental Sciences, Manipal Academy of Higher Education, Manipal University, Manipal, India.
Department of Biochemistry, Kasturba Medical College, Manipal Academy of Higher Education, Manipal University, Manipal, India.
Odontology. 2020 Oct;108(4):569-577. doi: 10.1007/s10266-020-00496-x. Epub 2020 Feb 17.
This case-controlled clinical trial was performed on the salivary 8-hydroxyguanosine (8-OHdG) levels in smokers and non-smokers with chronic periodontitis after non-surgical periodontal therapy. Subjects (N = 40) with periodontitis (smokers and non-smokers) and with clinically healthy conditions (smokers and non-smokers) were assigned to this study. At baseline, clinical periodontal parameters (plaque index, gingival index, pocket probing depth and clinical attachment levels) were evaluated. Saliva samples were obtained pre- and post-treatment to quantify the 8-OHdG levels using Elisa technique. Subjects diagnosed with chronic periodontitis with smoking habit (CPs) and non-smokers (CPns) received scaling and root planing. In clinically healthy subjects with smoking habit (CHs) and non-smokers (CHns), only oral hygiene tutoring was performed. All clinical measurements and salivary collection were repeated in a 3-month recall interval. Data were analyzed using Anova, Tukey post hoc test and Mann-Whitney 'U' tests (P < 0.05). At baseline, CPs and CPns groups showed significantly higher PI, GI, PD and CAL values than those of CHns and CHs (P < 0.001). Baseline salivary levels of 8-OHdG were significantly higher in CPs group (14.775 pg/mL) (P < 0.001) compared to the other groups. All clinical parameters in chronic periodontitis group improved at the 3rd month recall interval, however, with regards to 8-OHdG values, the CP smoker category still had a higher level compared to CP non-smoker. This study reflects an on-going periodontal destructive status in smokers and salivary 8-OHdG levels could be recognized as an oxidative biomarker for determining periodontal tissue destruction.
这项病例对照临床试验研究了非手术牙周治疗后慢性牙周炎患者和牙周健康者（吸烟者和不吸烟者）的唾液 8-羟基鸟嘌呤（8-OHdG）水平。将患有牙周炎（吸烟者和不吸烟者）和临床健康状况（吸烟者和不吸烟者）的受试者（N=40）纳入本研究。在基线时，评估了临床牙周参数（菌斑指数、牙龈指数、牙周袋探诊深度和临床附着水平）。在治疗前后采集唾液样本，使用 ELISA 技术定量 8-OHdG 水平。患有慢性牙周炎且有吸烟习惯的受试者（CPs）和不吸烟者（CPns）接受了洁治和根面平整。在有吸烟习惯的临床健康受试者（CHs）和不吸烟者（CHns）中，仅进行口腔卫生指导。所有临床测量和唾液采集均在 3 个月的随访间隔内重复进行。使用方差分析、Tukey 事后检验和 Mann-Whitney 'U'检验（P<0.05）分析数据。在基线时，CPs 和 CPns 组的 PI、GI、PD 和 CAL 值明显高于 CHns 和 CHs 组（P<0.001）。CPs 组的基线唾液 8-OHdG 水平明显高于其他组（14.775 pg/mL）（P<0.001）。慢性牙周炎组的所有临床参数在第 3 个月的随访间隔内均得到改善，但 CP 吸烟者组的 8-OHdG 值仍高于 CP 不吸烟者组。本研究反映了吸烟者持续的牙周破坏状态，唾液 8-OHdG 水平可作为确定牙周组织破坏的氧化生物标志物。
