Setor de Imunodiagnóstico do Laboratório de Micologia do Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Av Brasil 4365, Manguinhos, Rio de Janeiro 21045-900, Brazil.
Mycopathologia. 2010 May;169(5):359-63. doi: 10.1007/s11046-010-9276-7. Epub 2010 Feb 4.
This report describes the first isolation of Sporothrix globosa from a Brazilian patient. A 77-year-old woman was examined for sporotrichosis infection. Histopathological examination of skin biopsy revealed chronic granulomatous infiltrate with microabcess. Furthermore, S. schenckii-like yeasts were evident as demonstrated by PAS and Grocott stains. The fungus was identified based on colony morphology on Sabouraud Dextrose Agar slants, Potato Dextrose Agar, and Corn Meal Agar, microscopic morphology on slides cultures, and assimilation of different carbon sources. The species confirmation was made by molecular methodology.
本报告描述了首例从巴西患者中分离出球形孢子丝菌的情况。对一名 77 岁女性进行了孢子丝菌病感染检查。皮肤活检的组织病理学检查显示慢性肉芽肿性浸润伴微脓肿。此外，PAS 和 Grocott 染色显示类似申克孢子丝菌的酵母样真菌。根据沙氏琼脂斜面、土豆葡萄糖琼脂和玉米粉琼脂上的菌落形态、载玻片培养物上的微观形态以及不同碳源的同化作用，对真菌进行了鉴定。通过分子方法学进行了种的确认。
