Graduate School of Health Sciences, Okayama University, 5-1 Shikata-cho 2-chome, Kita-ku, Okayama-shi, Okayama 700-8558, Japan.
Ningyo-toge Environmental Engineering Center, Japan Atomic Energy Agency, 1550 Kamisaibara, Kagamino-cho, Tomata-gun, Okayama 708-0698, Japan.
J Radiat Res. 2021 Mar 10;62(2):206-216. doi: 10.1093/jrr/rraa129.
Radon inhalation activates antioxidative functions in mouse organs, thereby contributing to inhibition of oxidative stress-induced damage. However, the specific redox state of each organ after radon inhalation has not been reported. Therefore, in this study, we evaluated the redox state of various organs in mice following radon inhalation at concentrations of 2 or 20 kBq/m3 for 1, 3 or 10 days. Scatter plots were used to evaluate the relationship between antioxidative function and oxidative stress by principal component analysis (PCA) of data from control mice subjected to sham inhalation. The results of principal component (PC) 1 showed that the liver and kidney had high antioxidant capacity; the results of PC2 showed that the brain, pancreas and stomach had low antioxidant capacities and low lipid peroxide (LPO) content, whereas the lungs, heart, small intestine and large intestine had high LPO content but low antioxidant capacities. Furthermore, using the PCA of each obtained cluster, we observed altered correlation coefficients related to glutathione, hydrogen peroxide and LPO for all groups following radon inhalation. Correlation coefficients related to superoxide dismutase in organs with a low antioxidant capacity were also changed. These findings suggested that radon inhalation could alter the redox state in organs; however, its characteristics were dependent on the total antioxidant capacity of the organs as well as the radon concentration and inhalation time. The insights obtained from this study could be useful for developing therapeutic strategies targeting individual organs.
氡吸入激活了小鼠器官中的抗氧化功能，从而有助于抑制氧化应激诱导的损伤。然而，吸入氡后每个器官的具体氧化还原状态尚未报道。因此，在这项研究中，我们评估了浓度为 2 或 20 kBq/m3 的氡吸入 1、3 或 10 天后，小鼠各器官的氧化还原状态。通过对假吸入的对照小鼠数据进行主成分分析 (PCA)，用散点图评估抗氧化功能与氧化应激之间的关系。主成分 (PC) 1 的结果表明肝脏和肾脏具有高抗氧化能力；PC2 的结果表明大脑、胰腺和胃具有低抗氧化能力和低脂质过氧化物 (LPO) 含量，而肺、心脏、小肠和大肠具有高 LPO 含量但低抗氧化能力。此外，使用每个获得的聚类的 PCA，我们观察到在所有氡吸入组中与谷胱甘肽、过氧化氢和 LPO 相关的相关系数发生了变化。抗氧化能力低的器官中与超氧化物歧化酶相关的相关系数也发生了变化。这些发现表明，氡吸入会改变器官的氧化还原状态；然而，其特征取决于器官的总抗氧化能力以及氡浓度和吸入时间。本研究获得的见解可能有助于针对各个器官制定治疗策略。
