Grandjean Frederic, Gan Han Ming, Moumen Bouziane, Giraud Isabelle, Hatira Skander, Cordaux Richard, Austin Christopher M
Laboratoire Ecologie et Biologie des Interactions, Equipe Ecologie Evolution Symbiose, Unité Mixte de Recherche 7267 Centre National de la Recherche Scientifique, Université de Poitiers, Poitiers, France.
Deakin Genomics Centre, Deakin University, Geelong, 3220, Victoria, Australia.
Data Brief. 2020 Jan 27;29:105166. doi: 10.1016/j.dib.2020.105166. eCollection 2020 Apr.
The white-clawed crayfish () is an endangered species in Europe with limited genomic information. Despite its conservation status there is no transcriptomic data available for in public databases. The data here represents the first transcriptome profile of the white-clawed crayfish generated using Illumina stranded RNA sequencing. Pair-end reads were assembled with three separate transcriptome assemblers (Trinity, RNABloom, and RNASpades) followed by transcript assembly reduction and gene reconstruction using the EvidentialGene pipeline. The transcriptome was functionally annotated using InterProScan and genes coding for carbohydrate-active enzymes were identified through the dbCAN2 server. Raw fastq reads and the final version of the transcriptome assembly have been deposited in the NCBI-SRA (SRR10549898) and NCBI-TSA (GICG01) databases.
白爪小龙虾（）是欧洲的一种濒危物种，其基因组信息有限。尽管它处于受保护状态，但公共数据库中没有关于它的转录组数据。这里的数据代表了使用Illumina链特异性RNA测序生成的白爪小龙虾的首个转录组图谱。双末端 reads 使用三个独立的转录组组装器（Trinity、RNABloom 和 RNASpades）进行组装，随后使用 EvidentialGene 管道进行转录本组装缩减和基因重建。使用 InterProScan 对转录组进行功能注释，并通过 dbCAN2 服务器鉴定编码碳水化合物活性酶的基因。原始 fastq reads 和转录组组装的最终版本已存入 NCBI-SRA（SRR10549898）和 NCBI-TSA（GICG01）数据库。
