Li Cheng, Liu Xinyi, Ruan Hui, Zhang Jingyao, Xie Fengbin, Gai Junyi, Yang Shouping
Soybean Research Institute, National Center for Soybean Improvement, Key Laboratory of Biology and Genetic Improvement of Soybean (General, Ministry of Agriculture), State Key Laboratory of Crop Genetics and Germplasm Enhancement, Jiangsu Collaborative Innovation Center for Modern Crop Production, Nanjing Agricultural University, Nanjing, China.
Front Plant Sci. 2020 Jan 29;10:1714. doi: 10.3389/fpls.2019.01714. eCollection 2019.
WRKY protein is a unique transcription factor (TF) and plays an important role in the physiological processes of various stress responses and plant development. In this research, we obtained a WRKY TF gene from soybean by homologous cloning, and named it . is a nuclear protein containing a highly conserved WRKY domain and a CH zinc finger structure, and mainly expressed in roots, flowers and pods of soybean. The quantitative reverse transcription-PCR showed that was induced by phosphate starvation and salt stress. As compared with the wild type (WT), overexpression of increased the adaptability of transgenic to phosphate starvation, which might be related to the enhancement of lateral root development. The phosphorus concentration, fresh weight and dry weight of -overexpressing were higher than those of WT under Pi-sufficient or Pi-deficient condition. Meantime, the expression of phosphate-responsive genes was affected in transgenic Furthermore, improved the salt tolerance and changed fertility of transgenic . Under salt stress, we found the survival rate and soluble sugar content of transgenic were significantly higher than those of WT. In a conventional soil pot experiment, the transgenic produced shorter silique, less and larger seeds than WT, these might be due to partial abortion of pollens. The overall results showed that was not only involved in response to abiotic stress but also related to fertility, suggested that had an elaborate regulatory system in plants.
WRKY蛋白是一种独特的转录因子（TF），在各种应激反应和植物发育的生理过程中发挥着重要作用。在本研究中，我们通过同源克隆从大豆中获得了一个WRKY TF基因，并将其命名为 。 是一种核蛋白，含有高度保守的WRKY结构域和CH锌指结构，主要在大豆的根、花和豆荚中表达。定量逆转录PCR表明， 受磷饥饿和盐胁迫诱导。与野生型（WT）相比， 的过表达提高了转基因 对磷饥饿的适应性，这可能与侧根发育的增强有关。在磷充足或磷缺乏条件下，过表达 的植株的磷浓度、鲜重和干重均高于WT。同时，转基因 中磷响应基因的表达也受到影响。此外， 提高了转基因 的耐盐性并改变了其育性。在盐胁迫下，我们发现转基因 的存活率和可溶性糖含量显著高于WT。在常规土培实验中，转基因 产生的角果较短，种子数量较少且较大，这可能是由于部分花粉败育所致。总体结果表明， 不仅参与非生物胁迫响应，还与育性有关，提示 在植物中具有复杂的调控系统。
