Textile Research Division, National Research Centre, 33 El Bohouthst. (Former El Tahrir St.), Dokki, Giza P.O. 12622, Egypt.
High Institute of Applied Arts, the 5PthP District, Cairo, Egypt.
Int J Biol Macromol. 2020 May 15;151:821-829. doi: 10.1016/j.ijbiomac.2020.02.174. Epub 2020 Feb 18.
Green chemistry was used in nanostructures preparation to impart it amazing innovating application in the medical field. Herein we prepared novel, cost effective and ultra-safe antibacterial nanocomposite. This nanocomposite contains carboxymethylchitosan (CMCS) as safe reducing agent for gold nanoparticles (AuNPs) and polyvinyl alcohol (PVA) as nanofiber aiding material formation. The AuNPs has spherical shapes, its diameter ranged from 15 to 25 nm and uniform distributed within CMCS nanofibers. The optimum conditions for electrospinning were 10 wt% total solution contains 2 wt% CMCS and 8 wt% PVA. UV-vis, TEM and XRD were used to characterize AuNPs whereas FTIR and SEM were used to characterize nanofibers. Results showed that ultra-fine fibers were generated after addition of PVA to CMCS solution. Also, CMCS nanofibers containing AuNPs has excellent antibacterial activity towards tested bacteria. Finally, the cytotoxicity of CMCS has been evaluated through Cell viability assay, which confirm that the nanofiber composite is non-toxic and tissue compatible.
绿色化学被用于纳米结构的制备，使其在医学领域具有令人惊叹的创新应用。在此，我们制备了新颖、经济高效且超安全的抗菌纳米复合材料。该纳米复合材料包含羧甲基壳聚糖（CMCS）作为金纳米粒子（AuNPs）的安全还原剂和聚乙烯醇（PVA）作为纳米纤维辅助材料形成。AuNPs 呈球形，直径为 15 至 25nm，均匀分布在 CMCS 纳米纤维内。电纺的最佳条件是总溶液 10wt%，包含 2wt%CMCS 和 8wt%PVA。紫外可见光谱、TEM 和 XRD 用于表征 AuNPs，而 FTIR 和 SEM 用于表征纳米纤维。结果表明，向 CMCS 溶液中添加 PVA 后会生成超细纤维。此外，含有 AuNPs 的 CMCS 纳米纤维对测试细菌具有优异的抗菌活性。最后，通过细胞活力测定法评估了 CMCS 的细胞毒性，证实纳米纤维复合材料是无毒且与组织相容的。
